土人参多糖的抗氧化活性研究  

Study on the Antioxidation Activities of Polysaccharides from the Talinum Paniculatum

作　　者：潘廷啟[1] 文全泰[1] 黄礼德[1] 颜祖弟[1] 张照平[1] 蓝翠晓[1] 黄锁义[1] 

机构地区：[1]右江民族医学院,广西百色533000

出　　处：《时珍国医国药》2014年第1期30-32,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家中医药管理局“十二五”中医药重点学科建设项目(国中医药人教发[2012]32号);广西壮族自治区级大学生创新训练计划立项资助项目(No.QJCX201338);右江民族医学院民族医药协会资助项目

摘　　要：目的超声法提取土人参多糖,同时将粗多糖脱蛋白处理后,经处理得到的多糖进行抗氧化性研究,检测其抗氧化活性。方法通过单因素实验和正交实验,研究料液比、超声功率、超声提取温度和超声作用时间对土人参多糖提取效果的影响。制备土人参多糖,并对其清除DPPH、自由基、对羟基自由基以及对超氧阴离子自由基的作用进行试验,根据试验数据评价其抗氧化活性。结果超声提取法的优化工艺条件为:料液比(g∶ml)1∶20,提取温度80℃,超声功率180W,作用时间40 min。土人参多糖的得率为20.01%。抗氧化性试验显示土人参多糖对自由基的清除作用显著。结论土人参多糖具有较强的抗氧化活性,可为土人参药材资源的综合利用开发提供科学依据,土人参多糖具有一定的抗氧化能力。Objective To optimum the procedure of ultrasonic extracting polysaccharide from Talinum paniculatum and test the antioxidation of polysaccharides removed albumen by savage way. Methods Through single factor experiment and orthogonal ex- periment, research the solid - liquid ratio, ultrasonic temperature, ultrasonic power and ultrasonic action time to Talinum panicu- latum polysaccharide extraction influence effect. The experiment in the polysaccharide from Talinum paniculatum scavenging DP- PH, free radical, hydroxyl radical and superoxide radical was conducted to evaluate its antioxidant activity. Results The optimum ultrasonic extraction conditions： solid -liquid ratio（ g： ml） was 1： 20, temperature was 80~C, ultrasonic power was 180 W and ul- trasonic action time was 40 min. The extraction rate of po]ysaccharides was up to 20.01%. The experiment of antioxidation re- vealed that polysaccharides from stem of Talinum paniculatum had great effect of scavenging for radical. Conclusion The methods proved that the polysaccharides from stem of Talinum paniculatum had a powerful antioxidant activity, and it can be used for the extraction technology of polysaccharides from stem of Talinum paniculatum to provide theoretic basis, meanwhile, supplied scien- tific foundation for comprehensive development of medicinal materials.

关 键 词：关键词 土人参 正交设计 超声提取 多糖 抗氧化性 

分 类 号：R284.2[医药卫生—中药学]