广东地区猪源大肠杆菌耐药性及其系统发育群的研究  

Antimicrobial Resistance and Phylogenetic Analysis of E.coli Isolated from Swine in Guangdong Province

作　　者：郭潇木[1] 杨铜[1] 贺丹丹[1] 饶丽丽[1] 刘健华[1] 曾振灵[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《中国畜牧兽医》2014年第1期182-186,共5页China Animal Husbandry & Veterinary Medicine

基　　金：广东省自然科学基金团队项目(S2012030006590)

摘　　要：为分析广东地区猪源大肠杆菌耐药性及其系统进化背景,试验于2011年从患病猪分离出264株大肠杆菌,包括粪便源131株和肝源133株,采用琼脂稀释法测定18种抗菌药物的敏感性,多重PCR法确定系统进化关系,并对不同来源菌株的耐药性和系统进化背景进行分析比较。结果发现,大肠杆菌多重耐药现象严重,共存在128种耐药谱型。分离菌株对氨苄西林、复方新诺明、萘啶酸和四环素高度耐药,对黏菌素、头孢他啶和阿米卡星较敏感。种群进化分析结果表明,分离菌株主要分布在共生型的A组和B1组(91.66%),且不同来源菌株的种群进化分布差异不显著(P>0.05)。结合药敏试验结果发现,不同进化组别的分离菌株对特定抗菌药物的耐药性存在显著差异(P<0.05)。通过对大肠杆菌耐药性及其系统发育群进行综合分析,为兽医临床用药提供依据。To determine the antimicrobial resistance and the phylogenetic background of E.coli strains in Guangdong province, a total of 264 E.coli isolates were collected from diseased swine in 2011, which contained 131 strains from feces and 133 strains from liver. Antimicrobial susceptibility test was performed with agar dilution method and the phylogenetic background was determined by multiplex PCR. Statistical methods were used to analyze the relationship between antimicrobial resistance and the distribution of phylogenetic group among the E. coli strains isolated from feces and liver. The results showed that the multi-drug resistance （MDR） of the isolates was serious and there were 128 kinds of drug-resistant spectrum. The resistance rates of the isolates to ampicillin, sulfamethoxazole/trimethoprim, nalidixic acid and tetracycline were very high, and the isolates were sensitive to colistin, ceftazidime and amikacin. The majority of isolates belonged to phylogenetic groups A and B1 （91.66%）. There were no significant differences in distribution of phylogenetic group between the E.coli strains isolated from feces and liver （P＞0.05）, but the resistance rates to certain antimicrobials were significantly different in different phylogenetic groups （P＜0.05）. The study was to investigate antimicrobial resistance and the distribution of phylogenetic groups of the E.coli isolates so as to provide a foundation for the use of antimicrobials in veterinary clinical medication.

关 键 词：大肠杆菌 耐药性 系统进化分析 

分 类 号：S852.612[农业科学—基础兽医学]