血清游离DNA hTERT定量检测对胶质瘤的诊断意义  

Diagnostic significance of the quantity of serum DNA hTERT in glioma

作　　者：吕成林[1] 崔玉光[1] 施炜[2] 陈建[2] 

机构地区：[1]青岛市第八人民医院神经外科,266100 [2]南通大学附属医院神经外科

出　　处：《国际肿瘤学杂志》2014年第2期131-134,共4页Journal of International Oncology

摘　　要：目的 分析不同级别胶质瘤患者血清游离DNA中人类端粒酶逆转录酶（hTERT）的含量及其与临床病理特征之间的关系.方法 采集70例胶质瘤患者及22例正常对照者的外周血,用德国希尔登试剂盒提取DNA,实时荧光定量PCR检测血清DNA中hTERT含量,并比较其差异.随访胶质瘤患者的生存时间,比较血清DNA中hTERT含量与生存时间的关系.结果 胶质瘤患者血清DNA中hTERT含量显著高于正常对照组（107.41 ng/ml：34.79 ng/ml,P＜0.01）;70例胶质瘤患者中,高级别组血清DNA中hTERT含量显著高于低级别组（131.76 ng/ml：85.89 ng/ml,P=0.010）;术后随访1年,其中55例存活,10例死亡,5例失访,死亡组较存活组患者术前血清DNA中hTERT含量高（126.98 ng/ml：88.21 ng/ml,P=0.016）.受试者工作特征曲线分析血清DNA中hTERT含量,曲线下面积（AUC）为0.966.结论 检测血清游离DNA中hTERT含量有助于胶质瘤的筛查、早期诊断,并且在判断肿瘤恶性程度及预后方面具有重要意义.Objective To analyze the content of the cell-free DNA human telomerase reverse transcriptase （hTERT） in serum from patients with different grade glioma and its correlation with clinical-pathological parameters.Methods DNA was extracted from the serum of 70 patients with glioma and of 22 healthy controls with the QIAamp DNA Blood Mini Kit.The quantity of serum DNA hTERT was determined using real-time quantitative PCR and the difference was compared.The survival time of glioma patient was followed up and the relation between the quantity of serum DNA hTERT and survival time was analyzed.Results The level of serum DNA hTERT in glioma group was significantly higher than that in control group （107.41 ng/ml：34.79 ng/ml,P ＜0.01）.The level of DNA hTERT in serum of high grade glioma group was significantly higher than that of low grade group （131.76 ng/ml：85.89 ng/ml,P =0.010）.The patients were followed up for 1 year,of which 55 survived,10 patients died,5 patients were lost.The level of DNA hTERT in serum before operation in death group was higher than that in survival group （126.98 ng/ml：88.21 ng/ml,P =0.016）.The content of serum DNA hTERT was analyzed using ROC curve,and the area under the curve （AUC） was 0.966.Conclusion Quantification of serum DNA hTERT is valuable in screening,early diagnosis,determining the malignant degree and monitoring progression of gliomas.

关 键 词：DNA 肿瘤 聚合酶链反应 人类端粒酶逆转录酶 

分 类 号：R739.41[医药卫生—肿瘤]