IL-6和IL-8在正常胃组织、胃溃疡及胃癌组织中的表达  

Expressions of IL-6 and IL-8 in normal gastric tissue, gastric ulcer and gastric cancer

作　　者：赵志威[1] 朴大勋[1] 姜涛[1] 张哲男 王剑冰[1] 荆琼优 

机构地区：[1]哈尔滨医科大学附属第一医院普外科,150001

出　　处：《国际肿瘤学杂志》2014年第2期147-149,共3页Journal of International Oncology

基　　金：黑龙江省科学技术计划项目（LC02C15）

摘　　要：目的 探讨白细胞介素（IL）-6和IL-8在正常胃组织、胃溃疡及胃癌组织中的表达情况.方法 采用酶联免疫吸附法（ELISA）测定正常对照组（33例）、胃溃疡患者（30例）和准备手术的胃癌患者（52例）血浆中IL-6和IL-8的表达水平,并随访胃癌患者术后1周的IL-6、IL-8表达水平;免疫组织化学法检测胃癌周围正常组织（45例）、胃溃疡组织（35例）和胃癌组织（45例）标本中IL-6和IL-8的表达.结果 胃癌患者（术前和术后）血浆中IL-6、IL-8的表达明显高于胃溃疡和正常对照组（均P＜0.0l）,胃癌患者术后IL-6和IL-8水平较术前明显降低（P＜0.01）.癌周正常组织、胃溃疡和胃癌组织中IL-6和IL-8蛋白阳性表达率依次上升,且差异有统计学意义（x2=38.87,P＜0.01;x2=42.23,P＜0.01）.结论 IL-6和IL-8在胃癌患者血浆和胃癌组织中均表达上调,检测患者血浆和病理组织中的IL-6、IL-8水平有助于判断病情和评估预后.Objective To investigate the expressions of interleukin （IL）-6,IL-8 in normal gastric tissue,gastric ulcer and gastric cancer.Methods The plasma expressions of IL-6,IL-8 were determined by enzyme-linked immunosorbent assay in 33 normal controls,30 gastric ulcer patients and 52 gastric cancer patients （preparing for the surgery）,and the expressions of IL-6,IL-8 were followed up at the first week after operation.The expressions of IL-6,IL-8 in 45 normal mucosa tissues,35 gastric ulcer tissues and 45 gastric cancer tissues were determined by immunohistochemical method.Results The plasma expression levels of IL-6,IL-8 were significantly higher in patients with gastric cancer （pre-operation and post-operation） than normal controls and gastric ulcer patients（all P ＜0.01）,however the expression levels decreased after operation （P ＜0.01）.The positive expression rates of IL-6,IL-8 increased in turn in normal tissues,gastric ulcer tissues and gastric cancer tissues,and the differences were statistically significant （x2 =38.87,P ＜ 0.01 ; x2 =42.23,P ＜ 0.01）.Conclusion IL-6 and IL-8 are excessive expressed in the plasma and pathological tissue of gastric cancer patient,and the detection of IL-6 and IL-8 contributes to the judgment of patient＇s condition and prognosis estimation.

关 键 词：白细胞介素6 白细胞介素8 胃肿瘤 胃溃疡 

分 类 号：R735.2[医药卫生—肿瘤]