Apelin-13对实验性自身免疫性神经炎大鼠淋巴结中炎症因子TNF-α、IL-6和IFN-γ表达的影响  

Effect of Apelin-13 on the expression of TNF-α, IL-6 and IFN-γ in experimental autoimmune neuritis rats

作　　者：吴峥嵘[1,2] 谭利明[1] 赵鑫[1] 肖艳娇[1] 王志丽[1] 杨利[1] 

机构地区：[1]中南大学湘雅二医院神经内科,湖南长沙410011 [2]湖南省冷水江市人民医院神经内科,湖南冷水江417500

出　　处：《中国医药导报》2014年第4期17-20,共4页China Medical Herald

摘　　要：目的观察Apelin-13对实验性自身免疫性神经炎(EAN)大鼠淋巴结中炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和干扰素-γ(IFN-γ)表达的影响。方法采用周围神经髓鞘抗原(P257-81)注射入Lewis大鼠后肢足垫诱导EAN模型。Lewis雄性大鼠随机分为对照组、EAN模型组和Apelin处理组。Apelin处理组于免疫当天至第15天每天尾静脉注射Apelin-13(0.1 mg/kg)。观察各组大鼠发病情况和坐骨神经组织病理学变化,采用实时定量PCR和Western blot检测淋巴结中TNF-α、IL-6和IFN-γ表达。结果与EAN模型组比较,Apelin处理组大鼠最初发病时间明显延长,高峰期临床评分显著降低,炎症因子TNF-α、IL-6和IFN-γ的表达显著降低(均P<0.05)。结论 Apelin-13对实验性自身免疫性神经炎大鼠有治疗作用,其机制可能与下调TNF-α、IL-6和IFN-γ的表达有关。Objective To observe the effects of Apelin-13 on the expression of tumor necrosis factor-α （TNF-α）,interleukin-6 （IL-6） and interferon-γ （IFN-γ） of lymph node in experimental autoimmune neuritis （EAN） rats.Methods The rats models of EAN were established by injection of peripheral nerve myelin sheath antigen （P257-81） in the foot pad of male Lewis rats.The rats were randomly divided into the control group,EAN model group,and Apelin treatment group.The rats in Apelin treatment group were injected with Apelin-13 （0.1 mg/kg） by tail vein once daily from the 1st day to the 15th day.The clinical incidence and pathology in the rats were assessed.The expressions of TNF-α,IL-6 and IFN-γin lymph node were measured by real-time PCR and Western blot respectively.Results Compared with the EAN model group,the initial time of nervous symptom significantly increased,the maximal neurological score significantly decreased,the expressions of TNF-α,IL-6 and IFN-γ in lymph node significantly decreased in Apelin treatment group （all P ＜ 0.05）.Conclusion Apelin-13 can treat the EAN in EAN rats model,the mechanism of which may be related with down-regulation of TNF-oα,IL-6 and IFN-γ.

关 键 词：APELIN 实验性自身免疫性神经炎 肿瘤坏死因子-α 白细胞介素-6 干扰素-Γ 

分 类 号：R741.05[医药卫生—神经病学与精神病学]