CXCL10血清水平及单核苷酸多态性与慢性丙型肝炎患者发生甲状腺功能异常的相关性研究  

Association between serum levels and single nucleotide polymorphisms of CXCL10 and thyroid dysfunction in patients with chronic hepatitis C

作　　者：邵翠萍[1] 霍娜[1] 范晓红[1] 郑颖颖[1] 鲍毅[1] 李芳[1] 李敏然[1] 陆海英[1] 徐小元[1] 

机构地区：[1]北京大学第一医院感染疾病科,100034

出　　处：《传染病信息》2013年第6期348-350,354,共4页Infectious Disease Information

基　　金：国家"十二五"科技重大专项(2012ZX10002003-004-003);北京市科委2012年度科技计划重大项目(D121100003912-003)

摘　　要：目的研究CXCL10血清水平及单核苷酸多态性(single nucleotide polymorphisms,SNPs)与未接受干扰素(interferon,IFN)α治疗的慢性丙型肝炎(chronic hepatitis C,CHC)患者发生甲状腺功能异常的相关性。方法在前期已经进行甲状腺功能检测的312例未接受IFNα治疗的CHC患者中,筛选出与发生甲状腺功能异常的39例患者(甲状腺功能异常组)匹配的甲状腺功能正常患者39例(甲状腺功能正常组)。采用ELISA分别检测2组血清CXCL10水平;采用QIAamp DNA Blood Mini Kit提取2组患者基因组DNA,分别对CXCL10-135和CXCL10-1447基因片段进行PCR扩增和直接序列测定,应用BioEdit软件对样本序列进行分析。结果甲状腺功能异常组和正常组的血清CXCL10水平分别为(472.11±274.91)pg/ml和(516.39±272.40)pg/ml,2组比较差异无统计学意义。甲状腺功能异常组CXCL10-135的G、T等位基因频率分别为95.0%和5.0%,甲状腺功能正常组的G、T等位基因频率分别为96.2%和3.8%,2组比较差异无统计学意义。甲状腺功能异常组CXCL10-1447的A、G等位基因频率分别为41.7%和58.3%,甲状腺功能正常组的A、G等位基因频率分别为47.4%和52.6%,2组比较差异无统计学意义。甲状腺功能异常组CXCL10-135的GG、GT、TT基因型频率分别为93.4%、3.3%和3.3%,甲状腺功能正常组的GG、GT、TT基因型频率分别为92.3%、7.7%和0%,2组比较差异无统计学意义。甲状腺功能异常组CXCL10-1447的AA、AG、GG基因型频率分别为16.7%、50.0%和33.3%,甲状腺功能正常组的AA、AG、GG基因型频率分别为20.5%、53.9%和25.6%,2组比较差异无统计学意义。结论未接受IFNα治疗的CHC患者发生甲状腺功能异常与CXCL10血清水平和基因多态性位点-135、-1447无相关性。Objective To investigate the association between serum levels and single nucleotide polymorphisms （SNPs） of CXCL10 and thyroid dysfunction in patients with chronic hepatitis C （CHC） who were untreated with interferon （IFN） α. Methods Of 312 CHC patients untreated with IFNα, 39 were evaluated to have thyroid dysfunction using thyroid function tests （group with thyroid dysfunction）, and another 39 matched patients （group without thyroid dysfunction） were selected from the other 273 patients with-out thyroid dysfunction. Serum CXCL10 levels of the two groups were detected by ELISA. Genomic DNA were extracted by QIAamp DNA Blood Mini Kit; CXCL10-135 and CXCL10-1447 fragments were amplified by PCR and sequenced; BioEdit software was used to analyze the sequences. Results Serum CXCL10 levels of patients with and without thyroid dysfunction were 472.11±274.91 pg/ml and 516.39 ±272.40 pg/ml, respectively, and the difference between the two groups was not statistically significant. Frequen-cies of G and T alleles of CXCL10-135 were 95.0% and 5.0% in patients with thyroid dysfunction, and were 96.2% and 3.8% in patients without thyroid dysfunction, and the differences between the two groups were not statistically significant. Frequencies of A and G alleles of CXCL10-1447 were 41.7% and 58.3% in patients with thyroid dysfunction, and were 47.4% and 52.6% in patients without thyroid dysfunction, and the differences between the two groups were not statistically significant. Frequencies of GG, GT and TT genotypes of CXCL10-135 were 93.4%, 3.3% and 3.3% in patients with thyroid dysfunction, and were 92.3%, 7.7% and 0% in patients without thyroid dysfunction, and the differences between the two groups were not statistically significant. Frequencies of AA, AG and GG genotypes of CXCL10-1447 were 16.7%, 50.0% and 33.3% in patients with thyroid dysfunction, and were 20.5%, 53.9% and 25.6% in patients without thyroid dysfunction, and the differences between the two groups were not statistically sig-nificant .

关 键 词：丙型肝炎 慢性 甲状腺疾病 趋化细胞因子CXCL10 多态现象 单核苷酸 CHEMOKINE CXCL10 

分 类 号：R512.63[医药卫生—内科学] R581.3[医药卫生—临床医学]