New microbiota found in sputum from patients with community-acquired pneumonia  

作　　者：Chunyan Chen Tian Shen Fei Tian Ping Lin Qingtian Li Zelin Cui Yajuan Zhang Min Xue Jing Ye Xiaokui Guo Yuhua Zhou 

机构地区：[1]Department of Medical Microbiology and Parasitology/Department of Preventive Medicine, Shanghai Jiao Tong University School of Medicine,Shanghai 200025, China [2]Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China [3]Department of Laboratory Medicine, Ruij'in Hospital, Shanghai Jiao Tong Aniversity School of Me'dicine, Shanghai 200025, C~hina [4]Department of Respiratory, Shanghai Minhang Central Hospital, Shanghai 201100, China [5]Department of Emergency Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

出　　处：《Acta Biochimica et Biophysica Sinica》2013年第12期1039-1048,共10页生物化学与生物物理学报（英文版）

基　　金：This work was supported by grants from the National Natural Science Foundation of China （30770111, 30900051, 30970125）, the National Key Program for Infectious Diseases of China （2008ZX 10004-002, 2008ZX 10004-009, and 2009ZX10004-712）, and the Program of Shanghai Subject Chief Scientist （09XD 1402700）.

摘　　要：Community-acquired pneumonia （CAP） is a major concern in hospitals and the bacterial community of which has not been systemically discussed yet. Sputum from patients in the acute stages is a kind of accessible sample reflecting its fea- tures. In our study, we analyzed 45 sputum samples from 45 patients with CAP. Eighteen sputum samples from healthy people were chosen as the controls. Pyrosequencing of the 16s rDNA V3 hypervariable regions of aH the bacteria con- tained in the sputum was used as a culture-independent method to disclose the community constitution. Also, our published data for hospital-acquired pneumonia （HAP） in sputum was used for comparison. By pyrosequencing, 〉90,000 DNA reads were detected. After being analyzed by tools in the Ribosomal Database Project, the reads were clas- sified into five main phyla and 〉100 genera. At the phyla level, the reads＇ distribution of CAP is similar to that of healthy people and at genera level, the occurrence of each genus possesses their feature in three categories. Genera such as Streptococcus and Neisseria showed stability in their percentages, indicating that such genera are rarely affected by exogenous bacteria or antibiotics. The role of other genera such as Moraxella and Rothia in CAP should be emphasized. According to our analysis, the bacterial communities of CAP are with slight change when compared with those of healthy people, but have a large gap between HAP. Meanwhile, Rothia might be an important endogenous pneumonia-causing factor.Community-acquired pneumonia （CAP） is a major concern in hospitals and the bacterial community of which has not been systemically discussed yet. Sputum from patients in the acute stages is a kind of accessible sample reflecting its fea- tures. In our study, we analyzed 45 sputum samples from 45 patients with CAP. Eighteen sputum samples from healthy people were chosen as the controls. Pyrosequencing of the 16s rDNA V3 hypervariable regions of aH the bacteria con- tained in the sputum was used as a culture-independent method to disclose the community constitution. Also, our published data for hospital-acquired pneumonia （HAP） in sputum was used for comparison. By pyrosequencing, 〉90,000 DNA reads were detected. After being analyzed by tools in the Ribosomal Database Project, the reads were clas- sified into five main phyla and 〉100 genera. At the phyla level, the reads＇ distribution of CAP is similar to that of healthy people and at genera level, the occurrence of each genus possesses their feature in three categories. Genera such as Streptococcus and Neisseria showed stability in their percentages, indicating that such genera are rarely affected by exogenous bacteria or antibiotics. The role of other genera such as Moraxella and Rothia in CAP should be emphasized. According to our analysis, the bacterial communities of CAP are with slight change when compared with those of healthy people, but have a large gap between HAP. Meanwhile, Rothia might be an important endogenous pneumonia-causing factor.

关 键 词：16s ribosomal DNA MICROBIOTA community-acquired pneumonia PYROSEQUENCING SPUTUM 

分 类 号：S858.28[农业科学—临床兽医学] TU998.1[农业科学—兽医学]