PYROSEQUENCING

作品数：92被引量：353H指数：9
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TCERG1L hypermethylation is a risk factor of diabetic retinopathy in Chinese children with type 1 diabetes
《International Journal of Ophthalmology(English edition)》2024年第3期537-544,共8页Yu Qian Ying Xiao Qiu-Rong Lin Zhao-Yu Xiang Li-Pu Cui Jia-Qi Sun Si-Cong Li Xin-Ran Qin Hai-Dong Zou Chen-Hao Yang Pei-Yao Jin 
Supported by the National Key Research and Development Program of China(No.2016YFC0904800);National Natural Science Foundation of China(No.82101181);China Scholarship Council(No.201506230096);Shanghai Sailing Program(No.19YF1439700).
●AIM:To identify the differential methylation sites(DMS)and their according genes associated with diabetic retinopathy(DR)development in type 1 diabetes(T1DM)children.●METHODS:This study consists of two surveys.A to...
关键词：DNA methylation 850K array PYROSEQUENCING diabetic retinopathy type 1 diabetes CHILDREN 
An Alternative Low-Cost Strategy for Simultaneous Sensitive Detection of Adjacent ESR1 Mutations in Single Circulating Tumor Cell
《Journal of Analysis and Testing》2023年第1期89-100,共12页Chen Wang Jing-Jie Wang Xi-Ru Xue Yi-Yun Shen Bing-Jie Zou Shao-Hua Wang Qin-Xin Song Guo-Hua Zhou 
funded by the National Natural Science Foundation of China(81673390 and 61871403);Jiangsu Provincial key research and development program(BE2016745);The Open Project Program of MOE Key Laboratory of Drug Quality Control and Pharmacovigilance(DQCP2015MS02);sponsored by Qing Lan Project;Jiangsu Provincial Natural Science Foundation(BK20191322 and BK20180292);"Double First-Class"University project(CPU2018GY05 and CPU2018GY34);National Key R&D Program of China(2016YFA0201204);Jiangsu Provincial Science Fund for Distinguished Young Scholars(BK20180005);Jiangsu Provincial Medical Youth Talent Program(QNRC2016889);Innovation and Entrepreneurship Training Program for Undergraduate(202010316032S)
ESR1 mutation is of great clinical significance and being promoted as a marker of resistance to endocrine therapy in breast cancers.However,it is a challenging task to detect ESR1 mutations from traditional biopsies a...
关键词：ESR1 mutation Breast cancer Acquired resistance Single-cell amplification PYROSEQUENCING 
Direct evidence of microbiological water quality changes on bacterial quantity and community caused by plumbing system
《Journal of Environmental Sciences》2022年第6期175-183,共9页Jun Li Anran Ren Ed van der Mark Gang Liu 
the support from the National Key R&D program of China(No.2018YFE0204100);the National Natural Science Foundation of China(No.52022103);the National Natural Science Foundation of China for International Cooperation and Exchange(No.51820105011)。
Drinking water quality deteriorates from treatment plant to customer taps, especially in the plumbing system. There is no direct evidence about what the differences are contributed by plumbing system. This study compa...
关键词：Drinking water distribution system Plumbing system Microbiological water quality Sampling program 454 pyrosequencing 
The Nitrogen-Cycling Network of Bacterial Symbionts in the Sponge Spheciospongia vesparium
《Journal of Ocean University of China》2021年第4期999-1012,共14页HE Liming KARLEP Liisi LI Zhiyong 
Financial support from the National Natural Science Foundation of China(NSFC)(Nos.31861143020,41776138)was used to conduct this research and is greatly appreciated.
The microbes associated with sponges play important roles in the nitrogen cycle of the coral reefs ecosystem,e.g.,nitrification,denitrification,and nitrogen fixation.However,the whole nitrogen-cycling network has rema...
关键词：Spheciospongia vesparium bacterial symbionts 454 pyrosequencing functional gene analysis nitrogen-cycling net-work 
Looking into key bacterial proteins involved in gut dysbiosis
《World Journal of Methodology》2021年第4期130-143,共14页Xin-Yu Zeng Ming Li 
National Natural Science Foundation of China,No.81700457.
The gastrointestinal microbiota plays a pivotal role in health and has been linked to many diseases.With the rapid accumulation of pyrosequencing data of the bacterial composition,the causal-effect relationship betwee...
关键词：Gut microbiota PYROSEQUENCING Bacteria Protein IMMUNE DYSBIOSIS 
Predictive value of MGMT promoter methylation on the survival of TMZ treated IDH-mutant glioblastoma被引量：4
《Cancer Biology & Medicine》2021年第1期271-282,共12页Ruichao Chai Guanzhang Li Yuqing Liu Kenan Zhang Zheng Zhao Fan Wu Yuzhou Chang Bo Pang Jingjun Li Yangfang Li Tao Jiang Yongzhi Wang 
funded by the National Natural Science Foundation of China(Grant Nos.81903078 and 81773208);the Beijing Nova Program(Grant No.Z201100006820118);the National Key Research and Development Program of China(Grant No.2018YFC0115604);the National Natural Science Foundation of China(NSFC)/Research Grants Council(RGC)Joint Research Scheme(Grant No.81761168038);the Beijing Municipal Administration of Hospitals’Mission Plan(Grant No.SML20180501);the CAMS Innovation Fund for Medical Sciences(Grant No.2019-I2M-5-021);the Public Welfare Development and Reform Pilot Project of the Beijing Medical Research Institute(Grant No.JYY 2019-5)。
Objective:O6 methylguanine-DNA methyltransferase(MGMT)promoter methylation is a biomarker widely used to predict the sensitivity of IDH-wildtype glioblastoma to temozolomide therapy.Given that the IDH status has criti...
关键词：GLIOBLASTOMA O6methylguanine-DNA methyltransferase isocitrate dehydrogenase TEMOZOLOMIDE PYROSEQUENCING 
Interactive effects of elevation and land use on soil bacterial communities in the Tibetan Plateau被引量：4
《Pedosphere》2020年第6期817-831,共15页Yuanyuan YANG Yin ZHOU Zhou SHI Raphael A.VISCARRA ROSSEL Zongzheng LIANG Haizhen WANG Lianqing ZHOU Wu YU 
supported by the National Natural Science Foundation of China(Nos.41930754 and 41661061);the Natural Science Foundation of Tibet Autonomous Region of China(Nos.XZ2018ZRG-34(Z))
The Tibetan Plateau of China is uniquely vulnerable to the global climate change and anthropogenic disturbances.As soil bacteria exert a considerable influence on the ecosystem function,understanding their response to...
关键词：alpine ecosystem CLIMATE environmental controls PYROSEQUENCING soil microbe 
Comparison of soil bacterial diversity and community composition between clear-cut logging and control sites in a temperate deciduous broad-leaved forest in Mt. Sambong, South Korea
《Journal of Forestry Research》2020年第6期2367-2375,共9页Byeong-Ju Lee Soo Hyung Eo 
This study was carried out with the support of‘R&D Program for Forestry Technology(Project No.S211316L020130)'provided by Korea Forest Service.
Deforestation or clear-cut logging affects forest ecosystems,including soil microbial communities.The purpose of this study was to investigate the effects of clearcut logging on the soil bacterial community in a tempe...
关键词：Forest soil METAGENOMICS PYROSEQUENCING Soil physicochemical characteristics 
Analysis of PTEN Methylation Pattern by Pyrosequencing in Renal Cell Carcinoma
《Journal of Cancer Therapy》2020年第12期793-802,共10页Eurico Cleto Ribeiro de Campos Marcos Gonçalves Adriano Júnior Marcus Rivabem Winheski Renan Martins Ademar Lopes 
Objectives: We aimed to determine the frequency of PTEN (phosphatase with tensin homology deleted in chromosome 10) hypermethylation in r...
关键词：METHYLATION PTEN PYROSEQUENCING Renal Cell Carcinoma 
Blooms of Prorocentrum donghaiense reduced the species diversity of dinoflagellate community被引量：3
《Acta Oceanologica Sinica》2020年第4期110-119,共10页Huan Wang Zhangxi Hu Zhaoyang Chai Yunyan Deng Zifeng Zhan Ying Zhong Tang 
The National Natural Science Foundation of China under contract Nos 61533011 and 41776125;the NSFCShandong Joint Fund for Marine Ecology and Environmental Sciences under contract No.U1606404;the Scientific and Technological Innovation Project of the Qingdao National Laboratory for Marine Science and Technology under contract No.2016ASKJ02;the National Key R&D Program of China under contract No.2017YFC1404300;the Creative Team Project of the Laboratory for Marine Ecology and Environmental Science;Qingdao National Laboratory for Marine Science and Technology under contract No.LMEESCTSP-2018-1
Most of reported harmful algal blooms(HABs)of microalgae(75%)have been caused by dinoflagellates.Studies on the negative effects of HABs have generally focused on animals,valuable organisms in particular,and environme...
关键词：Prorocentrum donghaiense dinoflagellate community DIVERSITY PYROSEQUENCING East China Sea 
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