南极冰藻Chlam ydomonas sp.ICE-L RNA解旋酶CiDDX5的基因特征及其表达分析  

Characteristics and Expression Analysis of a RNA Helicase CiDDX5 Gene From the Antarctic Ice AlgaChlamydomonas sp.ICE-L

作　　者：宓妍妍[1,2] 王燕[1] 黄晓航[2] 刘晨临[2] 

机构地区：[1]山东轻工业学院食品与生物工程学院,山东济南250353 [2]国家海洋局第一海洋研究所,山东青岛266061

出　　处：《海洋科学进展》2013年第4期566-573,共8页Advances in Marine Science

基　　金：国家自然科学基金项目--RNA解旋酶在南极海冰衣藻冰冻环境适应中的作用机制(41276203)

摘　　要：DEAD-box RNA解旋酶能介导NTP依赖的双链RNA解旋,大量的研究表明其在植物的各种非生物胁迫的适应过程中发挥着重要作用。本研究从严格嗜冷的南极冰藻Chlamydomonas sp.ICE-L的EST序列中筛选并克隆得到了DEAD-box RNA解旋酶CiDDX5的全长基因,利用生物信息学软件对该基因编码的蛋白序列进行了研究,并通过实时定量PCR研究了在低温、高盐胁迫下CiDDX5基因表达量的变化。研究结果显示,DEAD-box RNA解旋酶CiDDX5基因的编码区长2 077bp,编码513个氨基酸。在以氨基酸序列构建的系统进化树中,CiDDX5序列和其他绿藻类的聚在一起,与莱茵衣藻,团藻和胶球藻的蛋白序列同源性依次分别为65%,64%和63%。实时定量PCR研究结果表明,在低温和高盐胁迫下,CiDDX5基因的表达量都有一定程度的提高,提示该基因的功能与南极衣藻适应低温、高盐的海冰环境的特性相关。DEAD-box helicase not only mediated NTP-dependent double-stranded RNA helix, but also plays an important role in the process of plant adaptation to various abiotic stresses. A DEAD-box RNA helicase CiDDX5 gene was identified from the EST library of the Antarctic ice alga, Chlarnydornonas sp. ICE-L. The complete sequence of the CiDDX5 gene contained an open reading frame of 2,077 bps encoding a RNA helicase of 513 amino acids. Phylogenetic analysis showed that the gene was homologous to the known green algae RNA helicase genes with identities of 65%, 64% and 63% to C. reinhardtii, Volvox carteri and Chlorella vulgaris respectively. The expressions of CiDDX5 gene were up-regulated under both the low temperature and high salinity stress conditions, which indicated the role of CiDDX5 gene in the regulation of abiotic stress adaptation of Antarctic ice alga Chlamydornonas sp. ICE-L.

关 键 词：南极冰藻 CHLAMYDOMONAS SP ICE-L DEAD-BOX RNA解旋酶 低温 高盐胁迫适应 

分 类 号：X834[环境科学与工程—环境工程]