IPL与595nmPDL对兔耳增生性瘢痕PCNA及TGF-β_1蛋白表达的影响  

Effects of IPL and 595nm PDL on the Expression of PCNA and TGF-β_1 in Hypertrophic Scar of Rabbit's Ears

作　　者：张幸存[1,2] 李大铁[1] 安丽莎[1] 孙娜娜[1] 

机构地区：[1]遵义医学院附属医院美容皮肤激光科,贵州遵义563000 [2]第三军医大学大坪医院整形美容科,重庆400042

出　　处：《中国皮肤性病学杂志》2014年第2期123-127,共5页The Chinese Journal of Dermatovenereology

基　　金：贵州省科技厅基金(黔科合SY字2008I3055)

摘　　要：目的探讨IPL与595nm PDL治疗兔耳增生性瘢痕的作用机制,比较两种治疗方法的差异。方法选用12只新西兰大耳白兔建立兔耳增生性瘢痕模型,随机分为A组和B组。A组左、右耳随机分为IPL治疗组(SI)和对照组(CI);B组左、右耳随机分为595nm PDL治疗组(SP)和对照组(CP)。治疗组在建模术后第5,7和9周时进行IPL和595nm PDL治疗,对照组不予治疗。每次治疗前及建模术后11周时进行肉眼观察并切取组织标本行HE染色、Masson染色和免疫组织化学方法测定PCNA蛋白和TGF-β1蛋白。结果经过治疗,瘢痕颜色变淡,厚度降低,质地变软;真皮厚度明显变薄,成纤维细胞、胶原纤维和微血管明显减少,胶原变稀疏,排列变规则;SI组变化更明显。从建模术后7周时开始,SI组HI值显著小于同期3组;SI组、SP组TGF-β1蛋白表达较同期CI组、CP组均显著降低。建模术后9,11周,SP组HI值显著小于同期CI组、CP组。建模术后11周,SI组、SP组较同期CI组、CP组胶原纤维蓝染浅且纤细,排列整齐有序;NA值明显减少;PCNA蛋白表达明显减少。以上差异均有统计学意义(P均<0.05)。结论 IPL和595nm PDL对兔耳增生性瘢痕有明显治疗作用,其治疗机理之一可能在于影响PCNA和TGF-β1蛋白表达,抑制成纤维细胞增殖,促进瘢痕萎缩,从而达到治疗目的。Objective To explore the mechanism of IPL and 595nm PDL on treating hypertrophic scars of rabbit＇s ears. Compare the effects of two treatment methods. Methods Twelve New Zealand white rabbits were used to establish the model for the hypertrophic ear scars. The rabbits were randomly divided into group A and group B ,with group A divided further into IPL treatment group ST and control group C ,and group B divided into 595nm PDL treatment group Sp and control group Cp. The treatment groups at the 5th,7th,9th, llth week after operation. The control groups were untreated. Morphological appearances of the hypertrophic scars were observed, and specimen of scar tissues were cut at the 5th,7th,9th, 11 th week after operation, HE stai ning, masson staining, immunohistoehemistry for the change of expression of PCNA protein and TGF-131 pro tein. Results The color of scars became lighter after the IPL or 595nm PDL treatment, the thickness re duced and the texture were softer. The dermis layer of the scar tissue was thinner, the fibroblasts, collagen fi bers and mierovessels decreased evidently. The range of the collagen fibers was sparse and orderly. Group Sl changed more significantly. From the 7th week after operation, compared with the groups CI, Sp and Cp, HI was reduced in the group Sl,the difference was significant. The TGF-β1 positive expressions in hypertrophic scars of the treatment group S and Sp were lower than the control group C and Cp, and the difference was significant. In the 9th, 1 l th week after operation, compared with the group CI and Cp, HI was reduced in the group Sp, these groups had significant difference. In the 1 lth week after operation, compared with the control group CI and Cp, collagen fibers became stained slightly and thinner, regularly arranged of the treatment group S1 and Sp, the numerical density on area were lower, the difference was significant, the expression ofPCNA protein in hypertrophic scars decreased evidently the difference was significant （ all P 〈 0.05 ）. Con- clusion I

关 键 词：强脉冲光 脉冲染料激光 增生性瘢痕 PCNA蛋白 TGF—β1 蛋白 

分 类 号：R739.5[医药卫生—肿瘤]