早期胃癌特征性miRNA筛选  

Screening of specific miRNA in early gastric cancer

作　　者：韩静[1] 余江流[2] 凌志强[1] 

机构地区：[1]温州医科大学检验科学生命科学学院,325035 [2]浙江省肿瘤医院浙江省肿瘤研究所遗传室

出　　处：《中华胃肠外科杂志》2014年第2期175-179,共5页Chinese Journal of Gastrointestinal Surgery

基　　金：卫生部科学研究基金.浙江省医药卫生重大科技计划（WKJ2010-2-004）

摘　　要：目的检测早期胃癌组织中微小RNA（miRNA）表达谱，筛选出早期胃癌的特征性miRNA。方法应用中通量基因芯片技术来检测5例早期胃癌组织及其癌旁组织标本中miRNA的表达。结果相对于癌旁组织，早期胃癌组织中共有36个miRNA表达下调，如miR-9．1、miR-103、miR-141等；12个miRNA表达上调，如miR．196a、miR．142．3p、miR-25等。结论在早期胃癌组织中表达异常的miRNA可能与胃癌发生发展有着一定的相关性。Objective To examine the micro-RNA （miRNA） expression profile in tissues of early gastric cancer and to screen the specific miRNA associated with gastric cancer. Methods Gene chip technology was used to detect the expression of miRNA in early gastric cancer tissues and adjacent normal tissues. Results Compared to adjacent normal tissues, a total of 36 miRNAs were down- regulated, such as miR-9-1, miR-103 and miR-141, while 12 miRNAs were up-regulated, such as miR-196a, miR-142-3p and miR-25, etc. Conclusion Abnormal miRNA expression level in early gastric cancer tissues may be associated to the development of gastric cancer.

关 键 词：胃肿瘤 早期 微小RNA 基因芯片 

分 类 号：R735.2[医药卫生—肿瘤]