USPIO联合MR活体动态示踪技术在EPCs移植脑卒中大鼠模型中的作用探讨  

Application of Ultrasmall Superparamagnetic Iron Oxide Particles Combined with MR scan in Labeling Endothelial Progenitor Cells at Transient middle cerebral arterial occlusion rats Modle

作　　者：侯唯姝[1] 徐丽艳[1] 余永强[1] 

机构地区：[1]安徽医科大学第一附属医院放射科,合肥230022

出　　处：《临床放射学杂志》2014年第2期293-297,共5页Journal of Clinical Radiology

基　　金：安徽省自然科学基金项目(编号:11040606Q22)

摘　　要：目的证实超微超顺磁性氧化铁颗粒(USPIO)作为细胞内对比剂标记大鼠骨髓源性内皮祖细胞(EPCs)的可行性,并以大鼠大脑中动脉阻塞(tMCAO)模型为载体,探讨MR动态示踪在干细胞移植在中的作用。方法多聚赖氨酸(PLL)为转染剂、USPIO为细胞内对比剂标记EPCs,于第1、3、5天分别计算细胞标记率并检测细胞活力。tMCAO大鼠30只,随机分为两组,分别经尾静脉移植磁性标记EPCs(实验组)及未标记EPCs(对照组),行MR动态扫描及普鲁士蓝染色。结果 USPIO-PLL能够成功标记EPCs,标记率达98%,标记组与未标记组间细胞活力无明显差异。MR T2WI序列动态扫描于脑梗死区与正常脑组织交界处的边缘可观察到连续的低信号,T2*map序列更为明显。普鲁士蓝染色结果与体外细胞染色结果相符。结论 USPIO可以成功标记EPCs,MR活体动态示踪能够成功监测到磁性标记细胞。Objective To explore of uhrasmall superparamagnetic iron oxide（USPIO） particles combined with MR in labeling endothelial progenitor cells （EPCs） at Transient middle cerebral arterial occlusion （tMCAO） rats. Methods Poly lysine （PLL） was used as the transfection agent （TA） to induct USPIO particles into EPCs in vitro. The positive labe- ling rate of cells and cell vitality were calculated at 1,3 and 5 days after transplant EPCs. tMCAO modle was successfully performed in 30 aduh SD rats. Magnetically labeled cells （the experimental group） and unlabled cells （the control group） were injected intravenously into the tMCAO rats through the tail vein. MR imaging and prussian blue staining were per- formed at different time points. Results USPIO PLL as an intracellular contrast agent could label EPCs successfully. The labeling rate can reach as high as 98% after 5 days. Trypan blue stain shows that USPIO has no significant effect on the bi- ological characteristics of EPCs. MR imaging showed significant low signal intensity at the outer boundary of ischemic area both on T2WI and T2 ＊ map sequences, especially on T2 ＊ map sequence. The ischemic area contained iron-positive cells showed on Prussian blue staining was corresponding to MR images. Conclusion PLL USPIO can label EPCs effectively, and has no significant effect on stem cell growth at an appropriate concentration. MR is feasible for in vivo tracking of EPCs labled with USPIO in tMCAO rats.

关 键 词：超小超顺磁性氧化铁 磁共振成像 内皮祖细胞 脑卒中 

分 类 号：R743.3[医药卫生—神经病学与精神病学]