Rab25蛋白在非小细胞肺癌中的表达及临床意义  

Expression of Rab25 in non-small cell lung cancer and its clinical significance

作　　者：周璞[1] 刁鑫伟[2] 汤金梁[2] 彭丽娜[1] 张洪川[1] 陈正堂[1] 

机构地区：[1]第三军医大学新桥医院肿瘤研究所,重庆400037 [2]第三军医大学新桥医院病理科,重庆400037

出　　处：《解放军医学杂志》2014年第2期157-160,共4页Medical Journal of Chinese People's Liberation Army

基　　金：重庆市科委重点攻关项目(CSTC 2011AB5032)~~

摘　　要：目的探讨Rab25在非小细胞肺癌(NSCLC)组织中的表达及其与肿瘤增殖、转移的相关性。方法收集2009年1月-2010年6月第三军医大学新桥医院61例NSCLC组织(鳞癌31例、腺癌26例、腺鳞癌4例)和40例癌旁组织标本作为研究对象。采用免疫组化法检测Rab25在组织标本中的表达情况;分析Rab25表达与临床病理特征(患者性别、年龄、吸烟史,肿瘤组织类型、分化程度、体积、TNM分期、淋巴结转移)的关系。结果 Rab25主要定位于细胞膜和细胞质中,在NSCLC组织的阳性表达率为93.4%,在癌旁组织的阳性表达率为27.5%(P<0.01)。Rab25的表达与肿瘤的TNM分期和大小相关(P<0.05)。结论 Rab25在NSCLC组织中的表达明显升高,NSCLC患者分期越晚、肿瘤直径越大,Rab25表达越高。Objective To assess the expression of Rab2S protein in non-small cell lung cancer （NSCLC）, and explore the correlation of its expression with tumor proliferation and metastasis. Methods Sixty-one cases of NSCLC specimens （31 cases of squamous cell carcinoma, 26 cases of adenocarcinoma, and 4 cases of adenosquamous carcinoma） undergone surgical treatment, and 40 specimens of adjacent normal lung tissues were obtained from Jan. 2009 to Jun. 2010 at Xingqiao Hospital of Third Military Medical University. Immunochemistry method of MaxVision was used to detect the expression of Rab2S in the specimens, and then the correlation of the expression with the clinicopathological parameters （patients＇ sex, age, smoking history＇, tumor type, differentiation, volume, TNM stage, lymph metastasis, etc.） was analyzed using statistical software SPSS 21.0. Results Rab2S protein was mainly expressed in cytoplasm and cell membrane. The positive rate of Rab2S in NSCLC was 93.4%, which was significantly higher than that in adjacent normal tissues （27.5%, P〈O.01）. The expression of Rab25 protein was significantly associated with the TNM stage and tumor size （P〈0.05）. Conclusions The expression of Rab25 is obviously higher in NSCLC than in the adjacent normal tissues, and the expression is associated with TNM stage and tumor size. Moreover, the later of the NSCLC stage, the larger of tumor size, and the higher of Rah25 expression will be in the NSCLC tissue.

关 键 词：癌 非小细胞肺 rab25蛋白质 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]