非小细胞肺癌组织中CXCR4和Nrf2的表达及其临床意义  

CXCR4 and Nrf2 expressions in non-small cell lung cancer and their clinical implications

作　　者：胡婷华[1] 姚煜[2] 于硕[3] 郭卉[2] 田涛[2] 韩丽丽[2] 王文娟[2] 郭倩倩[2] 王晶[2] 南克俊[2] 王淑红[2] 

机构地区：[1]西安交通大学医学院第一附属医院呼吸内科,陕西西安710061 [2]西安交通大学医学院第一附属医院肿瘤科,陕西西安710061 [3]西安交通大学医学院第二附属医院普通外科,陕西西安710004

出　　处：《南方医科大学学报》2014年第2期153-158,共6页Journal of Southern Medical University

基　　金：国家自然科学基金(81172361;81001090;81201923);中央高校基本科研业务费(xjj2010013);高等学校博士学科点专项科研基金(20110201120061);西安交通大学医学院第一附属医院科研基金(2011YK.12);西安交通大学基本科研业务费(08143072)~~

摘　　要：目的通过检测临床非小细胞肺癌(NSCLC)标本中CXCR4和Nrf2的表达情况,以探究两者在疾病发生发展中的重要作用及相关性,为肺癌诊断和治疗提供新的思路。方法应用免疫组化及Real-time PCR方法对资料统计完整的66例NSCLC标本检测CXCR4及Nrf2的表达情况。结果在NSCLC组织中,CXCR4与Nrf2蛋白及mRNA的表达量均明显高于正常组织(P<0.01)。CXCR4的阳性表达与较大的肿瘤体积(P=0.048)、肿瘤低分化(P=0.024)、较晚的TNM分期(P=0.018)、淋巴转移(P=0.004)及远处转移(P=0.016)相关。Nrf2的阳性表达与较大的肿瘤体积(P=0.008)、较晚的肿瘤TNM分期(P=0.028)、淋巴结转移(P=0.038)与远处转移(P=0.023)相关。进一步发现NSCLC组织中CXCR4与Nrf2的表达呈正相关(r=0.324,P<0.01),且CXCR4、Nrf2共同阳性表达与淋巴结转移及远处转移存在显著相关性。结论证明CXCR4和Nrf2的异常表达与NSCLC的进展、恶性生物学行为密切相关,且二者在NSCLC的发生发展中存在协同作用。Objective To detect the expressions of CXCR4 and Nrf2 in non-small cell lung cancer （NSCLC） tissues and analyze their association with the clinicopathological features of NSCLC. Methods We investigated the expressions of CXCR4 and Nrf2 in 66 NSCLC and corresponding distant normal tissue specimens using immumohistochemistry and real-time PCR. Results The expressions of CXCR4 protein and mRNA were significantly higher in NSCLC tissue specimens than in the distant normal tissues, while the expression of Nrf2 protein and mRNA increased significantly in NSCLC tissues compared to those in the distant normal tissues （P＆lt;0.01）. A high expression level of CXCR4 was positively correlated with a large tumor size （P=0.048）, poor differentiation （P=0.024）, advanced TNM stage （P=0.018）, lymph node metastasis （P=0.004）, and distant metastasis （P=0.016）. The expression of Nrf2 protein was positively correlated with a large tumor size （P=0.008）, advanced TNM stage （P=0.028）, lymph node metastasis （P=0.038）, and distant metastasis （P=0.023）. A strong correlation was found between CXCR4 and Nrf2 expressions in NSCLC tissues （r=0.324, P＆lt;0.01）, and the co-expression of CXCR4 and Nrf2 was strongly correlated with lymph node metastasis and distant metastasis. Conclusion Abnormal expressions of CXCR4 and Nrf2 may contribute to the progression and malignant biological behavior of NSCLC.

关 键 词：CXCR4 NFE2相关因子2 非小细胞肺癌 生物标记物 CXCR4 NRF2 

分 类 号：R734.2[医药卫生—肿瘤]