2-脱氧-D-葡萄糖增强紫杉醇抗小鼠乳腺癌移植瘤的作用  

2-Deoxy-D-glucose combined with Taxol inhibits VEGF expression and induces apoptosis in orthotopically transplanted breast cancer in C_3H mice

作　　者：张倩雯[1] 甘怀勇[2] 承泽农[2] 赵素容[1] 陈超[1] 蒋琛琛[1] 刘浩[1] 蒋志文[1] 

机构地区：[1]蚌埠医学院药学系//安徽省生化药物工程技术研究中心,安徽蚌埠233030 [2]蚌埠医学院第一附属医院病理科,安徽蚌埠233000

出　　处：《南方医科大学学报》2014年第2期193-196,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(81000992;81072207);安徽省高校省级自然科学研究项目(KJ2012A202)~~

摘　　要：目的：观察2-脱氧-D-葡萄糖（2-DG）联合紫杉醇（Taxol）对C3H小鼠乳腺癌移植瘤的抑制作用及其机制。方法建立肿瘤模型，待肿瘤体积达到100~300 mm3后，随机分为4组：对照组，2-DG组，Taxol组及2-DG和Taxol联用组。腹腔注射给药治疗18 d并观察记录肿瘤体积，每3 d 1次，绘制肿瘤生长曲线。接种后第19天处死动物，称量肿瘤质量，使用原位凋亡检测法（TUNEL）检测2-DG联合Taxol对肿瘤细胞凋亡的影响，免疫组化法检测血管内皮生长因子（VEGF）的表达。结果2-DG和Taxol联用组可明显抑制肿瘤生长（P〈0.05），单用Taxol组抑瘤率为36.97%，联用组为66.06%。TUNEL结果显示2-DG和Taxol联用组肿瘤组织中出现大量蓝黑色凋亡细胞，明显多于对照组，2-DG组与Taxol组，且VEGF的表达明显降低。结论2-DG联合Taxol对C3H小鼠移植性乳腺癌生长具有明显的抑制作用，这可能与诱导肿瘤细胞凋亡及抑制VEGF表达有关。Objective To investigate the antineoplastic effects of 2- Deoxy- D- glucose （2- DG） combined with Taxol on orthotopically transplanted breast cancer in C3H mice and explore the mechanism. Methods C3H mice bearing orthotopically transplanted breast cancer xenograft were randomly divided into 4 groups, namely the control group, 2-DG group, Taxol group, and 2-DG＋Taxol group. The corresponding drugs were administered intraperitoneally every 3 days for 18 consecutive days, and the tumor volume was measured every 3 days to draw the tumor growth curve. The mice were then sacrificed to measure the tumor weight on day 19 and examine tumor cell apoptosis with TUNEL assay and VEGF expression using immunohistochemistry. Results 2-DG combined with Taxol obviously suppressed the tumor growth with a tumor inhibition rate of 66.06% as compared to the rate of 36.97% in Taxol group. The combined treatment also caused more obvious cell apoptosis and significantly reduced VEGF expression in the tumor cells as compared with the other groups. Conclusion 2-DG can enhance the inhibitory effect of Taxol on orthotopically transplanted breast cancer xenograft in C3H mice probably by inducing tumor cell apoptosis and lowering VEGF expressions.

关 键 词：紫杉醇 C3H小鼠 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]