基质金属蛋白酶抑制剂GM6001抑制兔青光眼滤过性手术后瘢痕的形成  

GM6001 suppresses scar formation after glaucoma filtration surgery in rabbits

作　　者：申颖[1,2] 吴伟[1] 陆晓和[1] 郭文奇[3] 

机构地区：[1]南方医科大学珠江医院眼科,广东广州510282 [2]内蒙古医科大学附属医院眼科,内蒙古呼和浩特010050 [3]内蒙古医科大学第二附属医院急诊科,内蒙古呼和浩特010030

出　　处：《南方医科大学学报》2014年第2期241-245,共5页Journal of Southern Medical University

基　　金：内蒙古医学院青年基金(NY2009QN006);内蒙古自治区自然科学基金(2011MS1137)

摘　　要：目的探讨基质金属蛋白酶抑制剂GM6001在青光眼滤过术后滤过道瘢痕化中的作用。方法随机取青紫兰兔24只,双眼行抗青光眼小梁切除术,术后右眼结膜下注射GM6001作为实验组,左眼注射PBS作为对照组,1次/d,监测眼压,并通过免疫组化方法标记抗增殖细胞核抗原(PCNA)和α-平滑肌肌动蛋白(α-SMA),观察滤过道成纤维细胞增殖情况。结果术后7,14,21,28 d,实验组较对照组眼压低,术后各时间点实验组PCNA和α-SMA阳性细胞数低于对照组,均具有显著性差异(P<0.01)。结论 GM6001具有抑制兔眼滤过术后滤过道成纤维细胞过度增殖,减少瘢痕形成和延长功能性滤过泡存在时间的作用。Objective To study the effect of matrix metalloproteinases inhibitor GM6001 in suppressing scar tissue formation in the filtering passage after glaucoma filtration surgery. Methods Twenty- four pigmented rabbits （48 eyes） underwent trabeculectomy followed by subconjunctival injection of GM6001 in the right eye （treated eyes） and injection of PBS in the left eye （control） once a day. The intraocular pressure was monitored postoperatively and proliferating cell nuclear antigen （PCNA）- and α- smooth muscle actin （α- SMA）- positive cells in the filtering pathway were detected using immunohistochemistry. Results On postoperative days 7, 14, 21, and 28, the intraocular pressure was significantly lower in the treated eyes （GM6001） than in the control eyes （P＆lt;0.01）. The counts of PCNA-andα-SMA-positive cells were also significantly lowered in the treated than in the control eyes （P＆lt;0.01）. Conclusion GM6001 can inhibit excessive proliferation of the fibroblasts in the filtering pathway to suppress scar tissue formation and prolong the existence of the functional filtration bleb in rabbits.

关 键 词：基质金属蛋白酶抑制剂 GM6001 抗增殖细胞核抗原 Α-平滑肌肌动蛋白 GM6001 

分 类 号：R779.6[医药卫生—眼科]