L-苹果酸对吉富罗非鱼肠道结构功能及肝脏TCA循环效率的影响  

Effects of L-malic acid on intestinal histology and function,and TCA cycle efficiency of the liver of Gift tilapia( Oreochromis niloticus)

作　　者：李赞[1] 罗莉[1] 陈任孝[1] 白富瑾[1] 敬婷[1] 孙玉轩[1] 钱前[1] 

机构地区：[1]西南大学动物科技学院,淡水鱼类资源与生殖发育教育部重点实验室,水产科学重庆市市级重点实验室,重庆400715

出　　处：《水产学报》2014年第1期119-126,共8页Journal of Fisheries of China

基　　金：国家自然科学基金项目(31101909);重庆市自然科学基金(cstc2011jjA80021);中央高校基本科研业务费专项资金资助(XDJK2011C019)

摘　　要：实验选取体质量为（37．94±O．09）g吉富罗非鱼幼鱼420尾，随机分为7个处理（每个处理3个重复，每个重复20尾鱼），分别饲喂添加0（对照）、1．0、2．0、4．0、8．0、16．0和32．0g／kgL-苹果酸的饲料，进行为期154d的生长实验，研究L-苹果酸对罗非鱼肠道组织结构和功能及肝脏TCA循环效率的影响。结果发现，随着L-苹果酸水平的增加，前肠和中肠绒毛高度、密度、肌层厚度均在16．0g／kg组达到最大值且显著高于对照组（P〈0．05）；后肠绒毛高度、密度和肌层厚度逐渐降低（P〈0．05）。肠道淀粉酶和脂肪酶活性均在1．0g／kg组达到最大值且显著高于对照组（P〈0．05）；肠道蛋白酶、肝脏ATP含量随着L-苹果酸水平的增加逐渐升高（P〈0．05）；肠Na^＋-K^＋-ATP酶活力、肠表皮生长因子（EGF）和肠表皮生长因子受体（EGFR）含量在16．0g／kg组达到最高值；肝脏柠檬酸合酶活力在8．0g／kg到达最大值。研究表明吉富罗非鱼饲料中适量添加L-苹果酸（1．0～8．0g／kg），能改善罗非鱼前肠和中肠的形态结构，促进肠粘膜上皮细胞的增殖，提高肠消化和吸收能力，同时提高肝脏TCA循环蒲率．This experiment was conducted to study the effects of dietary L-malic acid levels on intestinal histology and function, and TCA cycle efficiency in liver of Gift tilapia. The experimental diets supplemented with 0（ control group） , 1.0,2.0,4.0,8.0,16.0 and 32.0 g/kg L-malic acid had been fed to juvenile tilapia with initial body weight of（37.94 ± 0.09） g for 154 days. 420 juvenile tilapia were randomly divided into seven groups and each group had three replicates （20 fishes for each replicate）. The results showed that：With the increment of L-malic acid supplement,the villus height, villus density and muscular thickness of foregut and midgut reached the highest in 16.0 g/kg group which was significantly higher than those in control group （P 〈 0.05 ）, and the villus height, villus density and muscular thickness of hindgut gradually decreased （P 〈 0.05）. Intestinal amylase and lipase activities reached the highest in 1.0 g/kg group which were significantly higher than those in control group （ P 〈 0.05 ）. The protease activity, and the content of ATP increased gradually with the increased level of L-malic acid （P 〈 0.05 ）. The activity of Na ~ -K ~ -ATPase, and the intestinal content of EGF and EGFR reached the highest in 16.0 g/kg group, meanwhile citrate synthase activity reached the highest in 8.0 g/kg group. This study indicates that in the dietary of Gift tilapia, a proper addition of L-malic acid （ 1.0 - 8.0 g/kg） can improve the morphology of foregut and midgut, promote the proliferation of intestinal mucosal epithelial cell, and improve the ability of intestinal digestion and absorption and the efficiency of liver TCA cycle.

关 键 词：吉富罗非鱼 L-苹果酸 肠道 肝 TCA循环 

分 类 号：Q591[生物学—生物化学] S965[农业科学—水产养殖]