一株鹅源巴氏杆菌的分离鉴定及耐药性分析  

Isolation and identification of Pasteurella multocida from goose and drug resistance analysis of the isolate

作　　者：刘红玉[1] 邵周伍林 李刚[1] 殷秀辰[1] 陈玉环[1] 吴晓颖[1] 刘思国[1] 张云[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001

出　　处：《中国兽医科学》2014年第2期119-123,共5页Chinese Veterinary Science

基　　金：现代农业水禽产业技术体系岗位科学家专项(CARS-43-10);国家自然科学基金面上项目(31072132)

摘　　要：对某种鹅场病死鹅进行剖解,肝涂片镜检后观察到革兰氏染色阴性短小杆菌,瑞氏-吉姆萨染色见两极浓染。用巴氏杆菌特异性引物进行PCR扩增,将扩增产物克隆至pMD18-T载体上,测序结果通过BLAST比对与多杀性禽巴氏杆菌的相似性为99.0%,确定其为多杀性巴氏杆菌。用16种常用抗菌药物的纸片法对该分离菌进行药敏试验,结果显示,该菌对四环素和万古霉素不敏感;对克林霉素、链霉素中度敏感;对内酰胺类(青霉素、氨苄西林)、大环内脂类(红霉素、克拉霉素)、喹诺酮类(环丙沙星)抗生素等高度敏感。用小白鼠和鹅进行动物回归试验,分为0.1mL/只、0.3mL/只和0.5mL/只剂量组,分别皮下接种TSB培养的病原菌,并分别在接种后6h和48h内出现死亡,死亡动物的剖检病变与巴氏杆菌感染的鹅相同,并在病变器官内分离出该病原菌。A pathogenic strain was isolated from the infected goose by routine bacterial isolation and identification technologies. The pathogen was identified as Pasteurella multocida anatis based on its mor-phological and culture characteristics and PCR results. The result of resistance test to 16 drugs showed that the bacterium was resistant to tetracycline and vancomycin,moderately sensitive to clindamycin and strep-tomycin, and highly sensitive to lactam, macrolide, quinolone antibiotics. In animal regression inoculation, 0.1 or 0.3 mL were inoculated in each mouse and 0.3 mL or 0.5 mL in each goose, and the animals ap- peared death in 6 h and 48 h, respectively. Autopsy lesions from death animal were the same as those of goose pasteurella disease and pathogenic bacteria recovered from corresponding organs.

关 键 词：多杀性巴氏杆菌 PCR检测 耐药性分析 分离鉴定 

分 类 号：S852.659.5[农业科学—基础兽医学]