干燥综合征患者心肺功能的变化与CD19^(+)CD24^(+)Brge及BTLA的相关性分析  

Changes of Heart and Lung Function in Patients with Sjogren Syndrome and Their Correlativity with CD19^(+)CD24^(+)Brge and BTLA

作　　者：王芳[1] 刘健[2] 叶英法 万磊[2] 曹永贺[1] 孙玥[1] 齐亚军[1] 

机构地区：[1]安徽中医药大学,安徽合肥230031 [2]安徽中医药大学第一附属医院,安徽合肥230031

出　　处：《风湿病与关节炎》2014年第2期5-10,共6页Rheumatism and Arthritis

基　　金：2012年国家科技支撑计划课题(2012BA126B02);国家自然科学基金2011年项目(2012-01至2015-12,81173211/H2708);国家中医药重点学科中医痹病学建设项目——国中医药发〔2009〕30号;国家中医药管理局科研课题专项基金(04-05LP27);安徽省科技厅科研计划项目(11010402170);安徽中医内科应用基础与开发研究省级实验室项目(科条〔2008〕150号)

摘　　要：目的:通过观察干燥综合征患者心、肺功能的变化与外周血CD19+CD24+Brge及BTLA表达的变化,探讨干燥综合征患者心、肺功能的异常与CD19+CD24+Brge及BTLA的关系。方法:采用超声心动图(UCG)检测64例干燥综合征患者(干燥综合征组)心功能变化,采用肺功能仪检测干燥综合征患者肺功能,采用流式细胞术检测外周血CD19+CD24+Brge及BTLA的表达频率,并与20例健康体检正常者(对照组)进行比较。结果:①干燥综合征组与对照组多普勒超声异常率比较,干燥综合征组多普勒超声心功能变化检测结果异常率为63.33%,对照组异常率为10.00%;干燥综合征组肺功能变化检测结果异常率为79.68%,对照组异常率为20.00%。两组比较,差异有统计学意义(P<0.01)。②与对照组比较,干燥综合征组心功能参数E峰、FS%、E/A比值均显著降低(P<0.01),A峰、LADd显著升高(P<0.01)。③与对照组比较,干燥综合征组肺功能指标IC、MVV、FEF25、FEF50、FEF75、PEF均明显降低(P<0.05或P<0.01)。④与对照组比较,干燥综合征组外周血BTLA占淋巴细胞比例明显降低(P<0.01),CD19+表达频率、CD24+表达频率、CD19++CD24+表达频率、CD19++BTLA占淋巴细胞比(%)、CD24++CD19+占淋巴细胞比(%)、CD24++BTLA占淋巴细胞比(%)显著升高(P<0.01)。⑤Spearman相关分析结果显示,干燥综合征患者心、肺功能与BTLA占淋巴细胞比(%)呈明显的负相关(P<0.05),E峰、LADd、PEF与CD19+表达频率、CD24+表达频率、CD19++BTLA占淋巴细胞比(%)、CD24++CD19+占淋巴细胞比(%)、CD24++BTLA占淋巴细胞比(%)均呈显著负相关(P<0.05);肺功能参数FEV1、FEF50与CD19+表达频率、CD24+表达频率、CD19++BTLA占淋巴细胞比(%)、CD24++CD19+占淋巴细胞比(%)呈显著正相关(P<0.05)。结论:干燥综合征患者在发生炎症的同时,其心、肺功能水平呈下降趋势,同时与外周血BTLA表达降低及CD19+CD24+Brge表达的升高呈相关性,提示Breg调控作用增强,引起�Objective：To study changes of heart and lung functions in patients with sj？gren syndrome and their correlativity with CD19＋CD24＋Brge and BTLA.Methods：Ultrasonic cardiogram （UCG） was used to detect the changes of heart function in 64 cases with sj？gren syndrome,pulmonary function apparatus was applied to test their pulmonary function,and flow cytometry was taken to detect the expression frequency of peripheral blood CD19＋CD24＋Brge and BTLA,which all were compared with those of the 20 healthy subjects （of the control group）.Results： ①The abnormal rate of Doppler ultrasound of the sjogren syndrome group was 63.33%and that of the control group was 10%;the abnormal rate of pulmonary function of the sjogren syndrome group was 79.68% and that of the control group was 20%,the difference between them being statistically signiifcant （P＆lt;0.01）. ②Compared with the control group,the heart function parameter E peak,FS%and E/A ratio of the sjogren syndrome group signiifcantly decreased （P＆lt;0.01）,while A peak and LADd signiifcantly increased （P ＆lt;0.01）.③ Compared with the control group,the lung function index such as MVV,FEF25,FEF50,FEF75 and PEF signiifcantly decreased in the sjogren syndrome group（P＆lt;0.05 or P＆lt;0.01）. ④In the sjogren syndrome group,compared with the control group,the ratio of peripheral blood BTLA to lymphocytes decreased signiifcantly （P＆lt;0.01）;the ratios of CD19＋expression frequency,CD24＋expression frequency,CD19＋＋CD24＋expression frequency and CD19＋＋BTLA frequency to lymphocyte（%）,CD24＋＋CD19＋to lymphocyte（%）,and CD24＋＋BTLA to lymphocyte（%） signiifcantly increased. ⑤The Spearman correlative analysis showed that in patients with sjogrem syndrome the heart-lung functions and the ratio of BTLA to lymphocyte （%）showed a signiifcant negative correlation. E peak,LADd,PEF and CD19＋expression frequency,CD24＋expression frequency,the ratios of CD19＋＋BTLA to lymphocyte（%）,CD24＋＋CD19＋t

关 键 词：干燥综合征 心功能 肺功能 CD19+CD24+Brge BTLA 

分 类 号：R593.2[医药卫生—内科学]