褪黑素联合顺铂对A549细胞凋亡相关基因的影响  

Effects of Melatonin Combined with Cisplatin on Apoptosis Related Gene of Human Lung Cancer Cell Line A549

作　　者：孙晓东[1] 王珺[1] 桑明[1,2] 王燕[1] 罗超[1] 余明华[3] 

机构地区：[1]湖北医药学院基础医学研究所,中国湖北十堰442000 [2]武汉大学动物实验中心/动物生物安全三级实验室,中国湖北武汉430071 [3]复旦大学附属浦东医院肿瘤科,中国上海201399

出　　处：《生命科学研究》2014年第1期50-54,共5页Life Science Research

基　　金：上海市浦东新区卫生系统学科带头人培养计划资助项目(PWRd2012-15);湖北省十堰市科技项目(十科发2010-047S;十科通[2006]18K33;十科发[2009]29S43)

摘　　要：观察褪黑素以及褪黑素联合顺铂是否可以抑制A549细胞增殖并促进细胞凋亡,探讨ERK1/2MAPK信号通路在这一过程中的作用,为肺腺癌的研究提供实验依据。用不同浓度的褪黑素、不同浓度的顺铂、褪黑素联合顺铂刺激体外培养的肺腺癌细胞系A549细胞,MTT法检测各组细胞的活性,运用Real-time RT-PCR检测凋亡相关基因Bcl-2、Bax、p53的表达水平,Western-blot检测ERK1/2MAPK的活性变化。结果:1)单纯使用褪黑素和顺铂均可抑制A549细胞增殖,褪黑素联合顺铂时,抑制作用更加显著,一定浓度的褪黑素可降低顺铂的使用浓度;2)未经处理的A549细胞Bcl-2/Bax很高,p53基因表达较低,经褪黑素以及褪黑素联合顺铂刺激后A549细胞中Bcl-2/Bax明显下降,p53基因表达明显升高,并有浓度依赖;3)经褪黑素和顺铂刺激后A549细胞中磷酸化ERK1/2MAPK水平明显降低。褪黑素联合顺铂可抑制A549细胞增殖并且诱导凋亡相关基因表达,褪黑素和顺铂对A549细胞的抑制作用可能与ERK1/2MAPK信号通路有关。To observe the effects of melatonin and melatonin combined with cisplatin on proliferation and apoptosis of human lung cancer cell line A549 and and the dependent signaling pathway. Lung cancer cell A549 were con-cultured with different concentrations of melatonin, different concentrations of cisplatin, dif- ferent concentrations of melatonin combined cisplatin for 24 h respectively. The activity of each group cells was detected by MTY assay;Real-time RT-PCR was used to analyze the expression of Bcl-2, Bax and p53; Western-blot was detected the changes of signal path ERK1/2MAPK. Results： 1） Melatonin and cisplatin significantly enhanced inhibition of cell proliferation of A549 cells, melatonin promoted the effects of cis- platin alone in A549 cells, reduced the using concentration of cisplatin; 2） The expression of Bcl-2/Bax was high but p53 gene was low in untreated A549 cells, the expression of Bcl-2/Bax decreased by stimulating with melatonin and eisplatin in A549 cells, expression of p53 gene after stimulation of the melatonin plus cisplatin rised significantly and concentration-dependent; 3） significantly lower after stimulation of melatonin and cisplatin proliferative and pro-apoptotic effects of cisplatin in A549 Phosphorylation levels of ERK1/2MAPK were in A549 cells. Melatonin potentiates the anti- cells by modulating ERK1/2MAPK signaling pathways.

关 键 词：褪黑素 顺铂 褪黑素联合顺铂 A549细胞 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]