纤维素酶法提取葶苈子多糖的工艺研究  

Research on the Process of Extraction Polysaccharide from Semen Lepidii Using the Cellulase

作　　者：颜祖弟[1] 李芸达[1] 张照平[1] 黄涛[1] 郑长慧 黄锁义[2] 

机构地区：[1]右江民族医学院临床学院,广西百色533000 [2]右江民族医学院药学院,广西百色533000

出　　处：《化学世界》2014年第2期80-82,128,共4页Chemical World

基　　金：国家中医药管理局"十二五"中医药重点学科建设项目(国中医药人教发[2012]32号)

摘　　要：优化纤维素酶法提取葶苈子多糖及其纯化。通过单因素实验和正交实验,研究酶解温度、提取时间、酶用量和pH值对葶苈子多糖提取率的影响,并经脱脂、热水抽提、乙醇沉淀、Sevage法脱蛋白得到葶苈子多糖。纤维素酶法提取的优化工艺条件为:pH值为5,加酶量4mL,酶解温度50℃,提取时间1h。在此条件下,葶苈子多糖的提取率平均为4.8%,RSD为0.12%。纤维素酶法提取葶苈子多糖省时高效,多糖的纯化方法简便,易操作,纯度高。To optimize the polysaccharide of Semen Lepidii and purify it. To research hydrolysis tempera- ture, extraction time, enzyme dosage and pH on the extraction yield of Semen Lepidii by single factor and orthogonal experiments, after degreasing, hot water extraction, ethanol precipitation, and sevage method of protein, the polysaccharide of Semen Lepidii can be got. The conditions of enzymatic extraction： pH 5, add 4 mL of the amount of enzyme hydrolysis, temperature 50℃, extraction time of 1 hours. Under these conditions, the extraction rate of polysaccharide Semen Lepidii average to 4.8 %. It is time-saving and effi- cient to optimize the Enzymatic extraction Semen I.epidii the polysaccharide, Polysaccharide purification method is simple and easy operation and high purity.

关 键 词：葶苈子 多糖 纤维素酶 正交设计 纯化 

分 类 号：R284[医药卫生—中药学]