上海市禽源阿贡纳沙门氏菌耐药性及脉冲场凝胶电泳分型研究  

Antimicrobial Resistance and Pulse Field Gel Electrophoresis Analysis of Salmonella agona Strains from Poultry in Shanghai

作　　者：杨筱薇[1] 旷代[1] 潘海建[1] 苏旭东[1] 吴丽萍 张建民[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]上海闪锦生物科技有限公司,上海201109

出　　处：《中国畜牧兽医》2014年第2期213-217,共5页China Animal Husbandry & Veterinary Medicine

基　　金：中国博士后科学基金第50批面上项目(2011M500781);中国博士后科学基金第5批特别资助项目(2010T50419)

摘　　要：为了解上海市2010-2011年零售禽肉及禽类粪便中阿贡纳沙门氏菌（S．agona）的流行、耐药性和分子分型情况，本研究通过对零售禽肉及禽类粪便中的沙门氏菌进行分离鉴定得到14株禽源阿贡纳沙门氏菌，通过K-B法进行耐药性分析，并应用脉冲场凝胶电泳（pulsefieldgelelectrophoresis，PFGE）技术对菌株进行分型研究。结果显示，14株禽源阿贡纳沙门氏菌对头孢替呋、头孢吡肟、头孢噻肟、头孢噻甲羧肟、甲氧氨苄嘧啶等敏感，对磺胺异恶唑等具有较强耐药性（21．4％），对链霉素中度敏感率较高（92．8％），PFGE分型分为6个基因型。结果表明部分菌株间具有高度的同源性，且耐药菌株与PF—GE型间存在相关性。To investigate the antimicrobial resistance and molecular types of S. agona strains isolated from poultry in Shang- hai from 2010 to 2011 ,K-B method recommended by WHO was used to detect 14 strains of S. agona from poultry. Results were analyzed according to the breakpoints of CLSI, PFGE was used for molecular typing. 14 strains were sensitive to 5 kinds of anti- biotics. High resistant to sulfisoxazole and high moderate sensitivity to streptomycin. 14 strains of S. agona were classified to 6 PFGE patterns. Some S. agona strains isolated from 2010 to 2011 were showed highly homology. Meanwhile,the relativity be- tween antimicrobial susceptibility and PFGE patterns was remarkable.

关 键 词：阿贡纳沙门氏菌 耐药性 脉冲场凝胶电泳 禽类 

分 类 号：S852.61[农业科学—基础兽医学]