逍遥散超临界CO_2提取工艺及提取物GC-MS特征图谱初步研究  

Study on supercritical CO_2 extraction of Xiaoyaosan and its GC-MS fingerprint

作　　者：左亚妹[1,2] 田俊生[1] 郭晓擎[1,2] 周玉枝[1] 高晓霞[1] 秦雪梅[1] 

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学化学化工学院,山西太原030006

出　　处：《中国中药杂志》2014年第4期674-678,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81102833;81173366);国家国际科技合作计划项目(2011DFA32630);国家"重大新药创制"科技重大专项(2012ZX09103201-035);山西省科技攻关项目(20130313015-1)

摘　　要：该文为了初步确定逍遥散超临界CO_2提取的最佳工艺条件,并建立提取物的气相色谱-质谱(GC-MS)特征图谱,以提取物得率为考察指标,采用正交试验设计方法,对提取压力、提取温度、CO_2流量、提取时间等因素进行考察;采用GC-MS对不同批次的提取物进行特征图谱分析。逍遥散超临界CO_2提取的最佳工艺条件为提取压力20 MPa,提取温度50℃,CO_2流量25 kg·h^(-1),提取时间3 h,提取物平均得率2.2%。GC-MS特征图谱中确定27个共有峰,占总峰面积的81.89%,其中指认出21个化合物,占提取物总量的53.20%。超临界CO_2萃取工艺合理可行,GC-MS方法准确可靠,重复性好,可用于逍遥散超临界CO_2提取物的制备及质量控制。To determine the optimum conditions of supercritical CO2 extraction of Xiaoyaosan, and establish its fingerprint by gas chromatography-mass spectrometry （GC-MS）, the yield of extract were investigated, an orthogonal test was used to quantify the effects of extraction temperature, pressure, CO2 flow rate and time, and fingerprint analysis of different batches of extracts were by GC- MS. The optimal extraction conditions were determined as follows： extraction pressure 20 MPa, extraction temperature 50 ℃, CO2 flow rate 25 kg . h-1 , extraction time 3 h, and average yield 2. 2%. The GC-MS fingerprint was established and 27 common peaks were found, whose contents add up to 81.89% of the total peak area. Among them, 21 compounds were identified, accounting for 53.20% of the total extract. The extraction process is reasonable and favorable for industrial production. The GC-MS method is accurate, reliable, reproducible, and can be used for quality control of supercritical CO2 extract from Xiaoyaosan.

关 键 词：逍遥散 低极性组分 超临界CO2提取 气相色谱-质谱 特征图谱 

分 类 号：TQ461[化学工程—制药化工]