缺氧时肺动脉内皮细胞生长状态的变化  

Effects of Hypoxia on the Growth of Pulmonary Artery Endothelial Cell.

作　　者：洪欣[1] 尹昭云[1] 孙兴斌[1] 昝俊平[1] 谢印芝[1] 吕永达[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《航天医学与医学工程》2000年第4期290-293,共4页Space Medicine & Medical Engineering

摘　　要：目的研究低氧时内皮细胞生长状态的变化 ,以揭示缺氧性肺水肿的发生机制。方法利用体外培养的肺动脉内皮细胞 (PAEC) ,观察了在缺氧条件 ( 3%O2 )下 ,PAEC的生长曲线、细胞周期及细胞内特征性蛋白因子的变化 ,对PAEC的生长状态进行了质和量综合评价。结果在观察的 48h范围内 ,缺氧对PAEC的生长数量无明显影响 (P >0 .0 5)。细胞的S期在缺氧 2h时出现了一过性的增加 ,同时G0 /G1 期出现了一过性的降低 (P <0 .0 5)。但随着缺氧时间的延长 ,PAEC的各个生长周期均无明显变化(P >0 .0 5)。因子Ⅷ相关抗原 (ⅧR :Ag)阳性细胞数在缺氧过程中均呈明显下降 (P <0 .0 5或P <0 .0 1 )。结论缺氧时 ,虽然PAEC在生长数量和生长周期上无明显变化 ,但生长质量出现了明显的下降 ,这将影响其功能状态 ,降低血管屏障作用的发挥。Objective To study the effects of hypoxia on the growth of pulmonary artery endothelialcell(PAEC). Method Growth number, growth cycle phase, and the characteristics of endothelial cells(factor Ⅷ related antigen) of PAEC were investigated during hypoxia using the PAEC cultured in vitro as a model. Result No difference was found between growth numbers of PAEC under hypoxia and that under normoxia conditions during the culture of 48 h(P>0.05). The numbers of positive immunofluoescence PAEC of factor Ⅷ related antigen in hypoxic groups were lower than those in normoxic groups.The cell cycle phase figures showed that the DNA synthetic phase (S phase) in hypoxia 2 hour group was increased significantly, while the pre synthetic phase(G 0/G 1) was decreased as compared with that of normoxic group. However, the S,G 2M(gap2 and mitotic phase) or G 1/G 0 stage of hypoxia for 12, 24 or 48 hours group all had no change as compared with those of normoxic group. Conclusion Growth of the PAEC changed significantly during hypoxia. Although the quantity and the growth cycle of PAEC showed little change, the quality was degraded seriously that its function as a permeability barrier would be affected.

关 键 词：缺氧 脉动脉 内皮细胞 生长 缺氧性肺水肿 发生机制 

分 类 号：R364.4[医药卫生—病理学] R852.11[医药卫生—基础医学]