坎地沙坦对人乳腺癌细胞株MCF-7生长抑制作用的研究  

The Effect of Candesartan on human breast cancer cell line MCF-7

作　　者：林淑慧[1] 谢国柱[1] 张静芳[1] 蔡小慧[1] 姚奇伟 孙权权[1] 王玮[1] 袁亚维[1] 

机构地区：[1]南方医科大学南方医院肿瘤放射治疗科,广州市510515

出　　处：《实用医学杂志》2014年第4期511-514,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:81272508);国家自然科学基金(编号:81302326);广东省自然科学基金(编号:S2011040003465);高等学校博士学科点专项科研基金(编号:20114433110015)

摘　　要：目的:探讨血管紧张素Ⅱ1型受体(AT1R)拮抗剂坎地沙坦对人乳腺癌细胞系MCF-7的生长抑制作用,为AT1R拮抗剂在乳腺癌中的应用提供实验依据。方法:噻唑蓝(MTT)比色法检测坎地沙坦对乳腺癌胞MCF-7细胞的生长抑制率,计算半数抑制浓度(IC50)。流式细胞仪分析坎地沙坦对MCF-7细胞周期和凋亡的影响。Western blot检测经坎地沙坦处理前后MCF-7细胞中野生型p53、survivin的蛋白表达差异。结果:坎地沙坦能够显著抑制乳腺癌细胞MCF-7的增殖,使细胞凋亡显著增强,促使细胞发生G1期周期阻滞,减小了S期细胞比例,上调其野生型p53和下调survivin蛋白表达水平。结论:坎地沙坦在体外抑制MCF-7细胞生长,诱导细胞发生周期阻滞和凋亡,机制可能与其调节p53、survivin蛋白的表达有关。Objective To investigate the proliferation inhibition of angiotensin Ⅱ 1 receptor （AT1R） antagonist candesartan on human breast cancer cell line MCF-7. Methods MCF-7 ceils were tested in vitro for cytotoxicity of candesartan with MTF method. The effect of candesartan on cell cycle and apoptosis of MCF-7 cells were evaluated by flow cytometry. Western Blot was applied to test the expression level of wild-type p53 and Survivin protein. Results After candesartan treatment, a dose- and time-dependent decreased proliferation in MCF-7 cells was observed. Induction of G1 cell cycle arrest and increased apoptosis in MCF-7 cells were also observed.Western blot assay showed that candesartan up-regulated wild-type P53 expression and down-regulated the Survivin expression in MCF-7 cells. Conclusions This study suggests that candesartan could inhibit the proliferation, induce G1 cell cycle arrest and apoptosis of MCF-Tcellsby regulating the protein expression level of gene p53 and Survivin.

关 键 词：乳腺肿瘤 坎地沙坦 细胞周期阻滞 凋亡 P53 SURVIVIN 

分 类 号：R737.9[医药卫生—肿瘤]