粗壮女贞水提物对金黄色葡萄球菌耐药株的抑菌效果及与抗生素的联合抑菌作用  

Study on the antibacterial activity of Ligustrum robustum combined with antibiotics to antibiotic resistant Staphylococcus aureus isolates

作　　者：杜晓昕[1] 叶倩[1] 陈凡[1] 左浩江[1] 黄梦娇 董泽丰 裴晓方[1] 

机构地区：[1]四川大学华西公共卫生学院(华西第四医院),四川成都610041

出　　处：《现代预防医学》2014年第5期894-897,901,共5页Modern Preventive Medicine

基　　金：粗壮女贞对肠道菌群的调节及减肥机制的研究(81273055)

摘　　要：目的研究粗壮女贞水提物对金黄色葡萄球菌临床耐药菌株的抑菌效果以及与抗生素的联合抑菌作用。方法采用纸片扩散法,观察粗壮女贞水提物对55株临床分离的金黄色葡萄球菌的抑菌效果,分别将青霉素、氨苄西林、红霉素和复方新诺明与粗壮女贞水提物合用,观察抑菌环的变化,用统计软件SPSS17.0进行比对分析。结果粗壮女贞水提物对55株金黄色葡萄球菌临床耐药菌株均有一定的抑制作用,平均抑菌环直径为(16.40±1.65)mm。青霉素、氨苄西林与水提物合用后的抑菌环直径比单用时增大,红霉素与水提物合用时抑菌环直径无明显变化,而复方新诺明与其合用时明显缩小。结论粗壮女贞水提物对不同耐药表型的金黄色葡萄球菌都有一定的抑制作用。水提物与青霉素类合用具有协同作用;与红霉素合用时呈无关作用;与复方新诺明呈拮抗作用。Objective To study the antimicrobial effects of aqueous extract from Ligustrum robustum and its activity combined with antibiotics against clinical drug-resistant strains of Staphylococcus aureus. Methods By using Kirby-Bauer Paper Disc Diffusion method, inhibitory test was carried out on 55 clinical drug-resistant strains of Staphylococcus aureus to observe the antibacterial activity of aqueous extract from Ligustrum robustum and the antibacterial activity combined with antibiotics, including penicillins, ampicillin, erythromycin and cotrimoxazole. The change of the diameters of the inhibition rings was observed and compared using SPSS17.0. Results The aqueous extract of Ligustrum robustum exhibited inhibition on all the 55 drug-resistant clinical isolates of Staphylococcus aureus. The average diameter of the inhibition rings was （16.40 ± 1.65） mm. When used in combination with the aqueous extract from Ligustrum robustum, the inhibition zones of penicillin, ampicillin, erythromycin and cotrimoxazole were observed larger, larger, unchanged and obviously smaller than those of the single antibiotics respectively. Conclusion The aqueous extract from Ligustrum robustum shows some antibacterial activity against Staphylococcus aureus in different drug resistance phe- notype. The aqueous extract from Ligustrum robustum shows synergistic action with penicillin and ampicillin. No cooperation or antagonism is observed between the aqueous extract and erythromycin, while antagonism is observed between the aqueous extract and cotrimoxazole.

关 键 词：粗壮女贞 金黄色葡萄球菌 抑菌 

分 类 号：R117[医药卫生—公共卫生与预防医学]