克百威和毒死蜱农药降解菌的筛选与鉴定  

Screening and identification of bacteria for degradation of pesticides carbofuran and chlorpyrifos

作　　者：王新[1] 陆佳靓[1] 刘丽[1] 崔丽[1] 梁吉艳[1] 

机构地区：[1]沈阳工业大学理学院,沈阳110870

出　　处：《生态学杂志》2014年第3期786-790,共5页Chinese Journal of Ecology

基　　金：辽宁省教育厅一般科研基金项目(L2012037);辽宁省高等学校杰出青年学者成长计划项目(LJQ2011010);中华环保环境保护基金会格平绿色行动辽宁环境科研教育"123工程"项目(CEPF2011-123-2-5)资助

摘　　要：采用多株细菌对克百威和毒死蜱农药分别进行降解实验,得到了具有优势的降解细菌并对其进行鉴定。结果表明,LLBK2、LLBK10对100 mg·L-1克百威的降解效果最好,48 h内的降解率分别为58.0%和60.0%,72 h内的降解率分别为82.8%和83.8%。LLBD2、LLBD4对100 mg·L-1毒死蜱的降解效果最好,48 h内的降解率分别为70.6%和61.5%,72 h内的降解率分别为92.3%和91.4%。同时对这4株细菌进一步鉴定,LLBK2、LLBK10、LLBD2均为蜡状芽胞杆菌(Bacillus circus),其16S rDNA基因序列在GenBank登录号分别为:JQ917438、HQ833023、JX847612,LLBD4为芽孢杆菌(Bacillus),其16S rDNA基因序列在GenBank登录号为JX010962。Several strains of bacteria were applied in the degradation experiments of carbofuran and chlorpyrifos, and the preponderant bacteria were identified by comparison. The LLBK2 and LLBK10 showed the best degradation effect on the 100 mg·L^-1 of carbofuran, with the degrada- tion rates of 58.0% and 60.0% in 48 hours, and 82.8% and 83.8% in the 72 hours, respec- tively. The LLBD2 and LLBD4 showed the best degradation effect on the 100 mg·L^-1of chlor- pyrifos, with the degradation rates of 70.6% and 61.5% in the 48 hours, and 92.3% and 91.4% in 72 hours, respectively. Through further microbiological identification, LLBK2, LL- BK10, LLBD2 could be classified as Bacillus circus. The accessing numbers of their 16S rDNA gene sequences in the GenBank were JQ917438, HQ833023, LLBD4 could be categorized as Bacillus, which has the GenBank and JX847612, accessing number respectively of JXO10962

关 键 词：细菌 克百威 毒死蜱 降解 

分 类 号：X172[环境科学与工程—环境科学] X592