RNA干扰靶向沉默Gankyrin对结肠癌增殖及侵袭的影响  

Effects of Gankyrin-siRNA on proliferation and invasion of human colonic cancer cell.

作　　者：宋海彬[1] 李建国[1] 毛晓光[1] 

机构地区：[1]黑龙江省医院,黑龙江哈尔滨150036

出　　处：《中国伤残医学》2014年第5期33-34,共2页Chinese Journal of Trauma and Disability Medicine

摘　　要：目的:观察靶向沉默Gankyrin基因对结肠癌细胞SW620在增殖和侵袭方面的影响。方法:通过体外合成针对Gankyrin基因的siRNA,通过脂质体转染SW620细胞,设立对照组检测细胞增殖、凋亡、细胞周期及侵袭能力。结果:细胞转染后,实验组的倍增时间为25.6±0.8小时,对照组倍增时间为21.7±0.6小时(P<0.01);细胞周期检测实验组的G1期细胞为68.3±0.7、S期细胞为29.6±0.9,对照组的G1期细胞为44.1±0.3、S期细胞为4.8±0.4(P<0.01);细胞凋亡检测实验组AnnexinV+PI-细胞比例为8.93±0.21,明显高于对照组1.31±0.06(P<0.01);细胞侵袭实验实验组细胞侵袭能率为34.2±0.3,明显低于对照组的53.9±0.6(P<0.01)。结论:siRNA干扰Gankyrin基因表达能够明显降低SW620的增殖和侵袭能力,提示Gankyrin可作为结肠癌基因治疗的潜在靶点。Objective：To probe the effect of Gankyrin gene silence by RNAi on the proliferation and invasion of human cancer of coloncell.Methods：SiRNA ,designed in vivo and targeting Gankyrin were transfected into SW 620 cells by lipofectamine 2000.The proliferationand invasion ability of SW620 cells are investigated in affects of cell population doubling time , cell cycle, apoptotic and invasion by comparing with control group.Results：Present research showed that Gankyrin-siRNA group had apparently longer cell population doublingtime [（25.6 ±0.8）h vs（21.7 ±0.6）h, P 〈0.01], higher cell apoptotic rate[（8.93 ±0.21）℅ vs（1.31 ±0.06）℅, P 〈0.01] andlower cell invasion rate [（34.2 ±0.3）℅ vs （53.9 ±0.6）℅, P 〈0.01].Meantime, Gankyrin-siRNA group dramatically inhibited SW620 cell at G1 phase in comparison with the control group.Conclusion：Gankyrin-siRNA can effectively decrease the proliferation andinvasion of human colonic cancer cell ,which reveals that Gankyrin might be a potential target for gene therapy of colon cancer .

关 键 词：结肠癌 Gankyrin RNA干扰 

分 类 号：R735.3[医药卫生—肿瘤]