江苏不同地区胃疾病相关幽门螺杆菌差异蛋白质的比较  

Proteomic analysis of Helicobacter pylori from patients with different gastric diseases in different regions of Jiangsu

作　　者：叶振宇[1] 李军成[1] 邢春根[1] 

机构地区：[1]苏州大学附属第二医院普通外科,江苏省苏州市215004

出　　处：《世界华人消化杂志》2014年第3期389-393,共5页World Chinese Journal of Digestology

摘　　要：目的:鉴定、比较江苏地区胃癌高、低发区胃癌与慢性胃炎相关幽门螺旋杆菌(Helicobacter pylori,H.pylori)差异表达蛋白质.方法:选取江苏省泰州市(胃癌高发区)和苏州市(胃癌低发区)胃癌和慢性胃炎患者胃镜下胃黏膜活检组织分离培养出的H.pylori,提取蛋白质后通过双向凝胶电泳进行分离,使用PDQuest软件对蛋白质点进行分析和比较,通过四极杆飞行时间电喷雾串联质谱对差异蛋白质点进行鉴定,并通过Mascot数据库检索.结果:所选H.pylori菌株总蛋白进行双向凝胶电泳后获得优质2-DE图谱,经Q-TOP鉴定为1号(巯基过氧化物酶)、3号(超氧物歧化酶)、4号(尿素酶α)、5号(核苷二磷酸激酶)、8号(分子伴侣dnaK)、9号(S-核糖基同型半胱氨酸裂解酶)、13号(无机焦磷酸酶)、14号(DNA引导的RNA聚合酶α)和16号(分子伴侣60×103)9个差异蛋白质点,其中3号、4号、8号和14号蛋白质点在胃癌菌株中均为高表达,1号和5号蛋白质点在慢性胃炎菌株中均为高表达,9号、13号和16号蛋白质点仅在一个地区存在差异表达.结论:胃癌高、低发区胃癌及相应地区慢性胃炎H.pylori蛋白质比较存在一定差异,但其中2/3的差异蛋白质是一致的,胃癌高发区H.pylori差异蛋白质高表达多于低发区,表明H.pylori在不同胃癌发病区致病机制相似,但在胃癌高发区作用强于低发区.AIM： To perform a proteomic analysis of He- licobacter pylori （H. pylori） from patients with different gastric diseases in different regions of Jiangsu. METHODS： H. pylori strains were isolated from the endoscopic biopsy specimens of gastric mu- cosa of patients with gastritis or gastric cancer from areas of high （Taizhou）and low （Suzhou） incidence of gastric cancer, and whole-cell pro- teins of H. pylori were extracted and character- ized by 2-dimensional electrophoresis （DE）. Different protein spots were analyzed using PDQuest analysis software, identified by electro- spray ionization quadruple time-of-flight mass spectrometry （ESI-Q-TOF-MS）, and searched us- ing the Mascot database. RESULTS： Nine differentially expressed proteins were identified, including four up-regu- lated in gastric cancer （superoxide dismutase, urease subunit alpha, chaperone protein dnaK and DNA-directed RNA polymerase subunit al- pha）, two up-regulated in chronic gastritis （ure- ase subunit alpha and nucleoside diphosphate kinase）, and three only expressed in strains from one region （S-ribosyl homocysteine lyase, inor- ganic pyrophosphatase and 60 x 103 chapero- nin）. CONCLUSION： There are proteomic differences between H. pylori isolates from patients with gastritis and gastric cancer in areas of high and low incidents of gastric cancer. The protein spots overexpressed in gastric cancer in the area with high incidence of gastric cancer are more than those in the area with low incidence of gastric cancer.

关 键 词：胃癌 慢性胃炎 幽门螺杆菌 蛋白质 

分 类 号：R735.2[医药卫生—肿瘤]