人大肠癌癌旁不同部位组织架构及相关分子差异  

Variance of crypt architecture and E-cadherin and PAR-3 expression in tissues at different distances from colorectal cancer lesions

作　　者：苏孟[1] 文彬[1] 胡丰良[1] 刘金元[1] 

机构地区：[1]广州中医药大学脾胃研究所,广东省广州市510405

出　　处：《世界华人消化杂志》2014年第3期444-449,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81173257~~

摘　　要：目的:观察在距大肠癌3个不同部位的癌旁组织隐窝微架构的变化及其相关上皮细胞特征分子E-cadherin和PAR-3表达分布差异.检测E-cadherin、PAR-3在距大肠癌病灶不同距离的癌旁组织中的表达及分布差异,探讨其对大肠癌发生的意义.方法:从距离大肠癌病灶近端10、5、2 cm处获取组织样本,并分别命名为1、2、3号位组织样本,观察3个部位组织隐窝微架构变化规律,并采用免疫组织化学(SP法)和半定量法Western blot检测1、2、3号位组织E-cadherin、PAR-3表达分布变化.结果:随着距离肿瘤病灶越来越近,组织隐窝微架构破坏(隐窝微架构不整齐,上皮缺损)愈来愈明显,免疫组织化学及Western blot实验表明,随着组织越来越靠近病灶,上皮细胞表面E-cadherin和PAR-3表达量依次递减,分布由细胞膜转向细胞浆愈来愈明显.结论:在大肠癌组织中,越靠近病灶的癌旁组织,隐窝微架构的变化越明显,甚至完全消失,而隐窝上皮细胞中E-cadherin和PAR-3表达也逐渐减弱,且分布由细胞膜逐渐转向细胞浆.AIM： To observe the variation of crypt architec- ture, expression and distribution of E-cadherin and PAR-3 expression in tissues at different dis- tances from colorectal cancer lesions. METHODS： Tissue samples at 10, 5 and 2 cm from the tumor lesion were collected. The varia- tion of crypt architecture was observed among the three groups. E-cadherin and PAR-3 expres- sion was detected by immunohistochemistry and Western blot. RESULTS： With the distance getting closer to the tumor lesion, crypt architecture was destroyed more and more obviously. Cell crypts were irregularly arranged, and some of them disappeared. With the distance getting closer to the tumor lesion, the expression of E-cadherin and PAR-3 decreased progressively, and E-cad- herin and PAR-3 translocated gradually from the plasma membrane to the cytoplasm. CONCLUSION： With the distance getting closer to the tumor lesion, crypt architecture was de- stroyed more and more obviously, and some crypts disappeared; E-cadherin and PAR-3 ex- pression in crypt epithelial cells decreased pro- gressively and gradually translocated from the plasma membrane to cytoplasm.

关 键 词：E-CADHERIN PAR-3 隐窝 大肠癌 癌旁组织 

分 类 号：R735.34[医药卫生—肿瘤]