肝内型门脉高压大鼠门静脉超微结构及生物力学特性的改变  

Changes in ultrastructure and biomechanical properties of the portal vein in rats with intrahepatic portal hypertension

作　　者：文彬[1] 邓鑫[1] 陈然[1] 彭佩纯 

机构地区：[1]广西中医药大学附属瑞康医院,广西壮族自治区南宁市530000

出　　处：《世界华人消化杂志》2014年第4期541-545,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81160433;广西科技厅重点课题基金资助项目;No.2011GXNSFD018035;广西卫生厅重点课题基金资助项目;No.广重20122031;中南大学博士后科学基金资助项目;No.2013M531816~~

摘　　要：目的:观察肝内型门脉高压大鼠门静脉超微结构及生物力学特性,为进一步探索门脉高压病理机制提供实验依据.方法:四氯化碳皮下注射诱导肝内型门静脉高压大鼠模型,生物力学技术测定门脉高压形成中门静脉在体直径、纵向拉伸应力-应变曲线及残余应力大小,透视电镜下观察门静脉超微结构.结果:与正常组大鼠相比,门脉高压组大鼠门静脉体外直径显著增粗(1.53 mm±0.28 mm vs 1.14 mm±0.17 mm,P<0.01);门静脉超微结构发生显著改变;纵向拉伸应力-应变曲线较陡峭,整个拉伸应变过程耗时短,50%应变时应力是1.04 N±0.13 N,显著大于正常组(0.51N±0.08 N,P<0.01);门静脉张开角的弧度及组内波动幅度较正常组显著增大(110.34度±8.09度vs 79.93度±2.44度,P<0.01).结论:肝内型门脉高压大鼠门静脉管壁存在异常重构,其顺应性下降,门静脉呈周向和轴向高应力的生物力学特性改变.AIM： To observe the ultrastructure and biome- chanical properties of the portal vein in rats with intrahepatic portal hypertension to provide an experimental basis for further exploring the pathogenesis of portal hypertension. METHODS： Subcutaneous injection of carbontetrachloride was performed to induce intrahe- patic portal hypertension in rats. Biomechanical methods were used to determine portal vein diameter, longitudinal tensile stress-strain curve and the size of the residual stress during portal hypertension formation. Portal vein ultrastruc- ture was observed by electron microscopy.RESULTS： Compared with normal rats, the portal vein diameter was significantly greater in rats with portal hypertension （1.53±0.28 mm vs 1.14 ± 0.17 mm, P 〈 0.01）; portal vein ultra- structure changed significantly; longitudinal tensile stress - strain curve was steeper; the time consumed for the whole process of tensile strain was shorter; the stress at 50% strain was signifi- cantly larger （1.04 ± 0.13 N vs 0.51 ± 0.08 N, P 〈 0.01）; the opening angle of the portal vein and arc fluctuations increased significantly （110.34°±8.09） vs 79.93° ± 2.44°, P 〈 0.01）.CONCLUSION： Rats with intrahepatic portal hypertension have abnormal portal vein wall remodeling, decreased portal vein compliance, and changes in circumferential and axial biome- chanical characteristics.

关 键 词：门脉高压 超微结构 应力-应变 残余应力 顺应性 

分 类 号：R575.21[医药卫生—消化系统]