葛根芩连汤对高脂饮食诱导大鼠非酒精性脂肪性肝炎的影响  

Effects of Gegen Qinglian Tang on high-fat diet-induced nonalcoholic steatohepatitis in rats

作　　者：续畅[1] 王允亮[2] 张慧芹[1] 刘泽洲[1] 郝钰[1] 牛建昭[1] 李军祥[2] 

机构地区：[1]北京中医药大学基础医学院,北京市100029 [2]北京中医药大学东方医院消化科,北京市100078

出　　处：《世界华人消化杂志》2014年第4期546-552,共7页World Chinese Journal of Digestology

基　　金：高等学校学科创新引智计划基金资助项目;No.B07007~~

摘　　要：目的:探讨葛根芩连汤对非酒精性脂肪性肝炎的干预作用.方法:高脂饲料喂养SD大鼠以制备非酒精性脂肪性肝炎模型,各给药组在造模的同时进行灌胃给药,持续8 wk后取材,血清用比色法对谷草转氨酶(aspartate aminotransferase,AST)、谷丙转氨酶(alanine transaminase,ALT)、总胆固醇(cholesterol total,CHO)、低密度脂蛋白((low density lipoprotein,LDL)、高密度脂蛋白(high density lipoprotein,HDL)、空腹血糖(fasting plasma glucose,FPG)的含量进行检测,用放免法对空腹胰岛素(fasting insulin,F I N S)的含量进行检测,并进行胰岛素抵抗指数(homeostatic model assessment of insulin resistance,HOMA-IR)计算;肝组织制成石蜡切片及冰冻切片进行HE及油红O染色,并根据"非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)活动度积分"对各组肝组织进行NASH评估.结果:与空白组比较,NASH大鼠血清中A S T、A LT、C H O、L D L的含量显著升高(P<0.05或P<0.01),H D L的含量显著降低(P<0.01),HOMA-IR指数显著升高(P<0.05),葛根芩连汤可显著抑制高脂饲料喂养SD大鼠过程中血清AST、ALT、CHO、LDL含量(P<0.01)及HOMA-IR指数的升高(P<0.05)以及HDL含量的降低(P<0.01);HE染色、油红O染色及NAFLD活动度积分结果显示,葛根芩连汤可显著抑制NASH及相关病理变化的发生发展.结论:葛根芩连汤对非酒精性脂肪性肝炎有一定的干预作用,并可改善胰岛素抵抗.AIM： To explore the effects of Gegen Qinglian Tang on high-fat diet-induced nonalcoholic steatohepatitis（NASH） in rats. METHODS： SD rats were fed a high-fat diet to induce nonalcoholic steatohepatitis. Different doses of Gegen Qinglian Tang were adminis- tered intragastrically when the rats were fed the high-fat diet. Blood samples were harvested after 8 wk to detect aspartate aminotransferase （AST）, alanine transaminase （ALT）, cholesteroltotal （CHO）, low density lipoprotein （LDL）, high density lipoprotein （HDL）, fasting plasma glucose （FPG）, and fasting insulin （FINS）. Liver tissue samples were taken for hematoxylin-eosin （HE） staining and oil-red O staining. In addition, nonalcoholic fatty liver disease （NAFLD） activity score （NAS） and homeostatic model assessment of insulin resistance （HOMA-IR） were assessed. RESULTS： Compared with the model group, the levels of serum AST, ALT, CHO, LDL and HOMA-IR were significantly decreased and the levels of serum HDL significantly increased in the high dose Gegen Qinlian group. The results of HE staining, oil-red O staining and NAS as- sessment showed that Gegen Qinlian Tang could significantly inhibit the development of NASH. CONCLUSION： Gegen Qinglian Tang has thera- peutic effects against NASH in SD rats, and it can improve insulin resistance.

关 键 词：结论 葛根苓连汤对非酒精性脂肪性肝炎有一定的干预作用.并可改善胰岛素抵抗 

分 类 号：R575.1[医药卫生—消化系统]