阿司匹林抗高糖诱导的内皮细胞衰老的作用  

Aspirin Attenuates High Glucose-induced Senescence in Human Umbilical Vein Endothelial Cells

作　　者：伊桐凝[1] 张锦[2] 于世家[1] 

机构地区：[1]辽宁中医药大学附属医院内分泌科,辽宁省沈阳市110031 [2]中国医科大学附属第一医院内分泌科,辽宁省沈阳市110001

出　　处：《中国动脉硬化杂志》2014年第1期27-31,共5页Chinese Journal of Arteriosclerosis

基　　金：辽宁省博士启动基金(20121101)

摘　　要：目的探讨阿司匹林是否具有抗高糖诱导的内皮细胞衰老的作用及其机制。方法人脐静脉内皮细胞(HUVEC)分别于正常糖浓度培养液(5.5 mmol/L)、高糖培养液和高糖(33 mmol/L)+阿司匹林(0.01、0.1、1及3 mmol/L)培养液中培养48 h,观察细胞形态、采用β-半乳糖苷酶染色鉴定衰老细胞,PCR-ELISA检测端粒酶活性,流式细胞仪检测细胞周期和细胞内活性氧水平。结果高糖培养液作用HUVEC 48 h后,细胞呈现衰老状态,β-半乳糖苷酶染色阳性细胞数明显增加,端粒酶活性明显减低,细胞周期停滞于G0/G1期,S期显著减少,细胞内活性氧水平显著增加。0.01、0.1和1 mmol/L阿司匹林作用后细胞形态改善,β-半乳糖苷酶染色阳性细胞数显著减少,端粒酶活性增强,S期细胞显著增加,细胞内活性氧水平降低(P<0.05)。而3 mmol/L阿司匹林作用后与高糖组相比差异无显著性。结论高糖环境下阿司匹林(0.01、0.1和1 mmol/L)具有抗内皮细胞衰老的作用,其作用机制可能与氧化应激有关。Aim To investigate the effect and mechanism of aspirin on human umbilical vein endothelial cells （HUVEC） senescence exposed to high glucose condition. Methods The cultured HUVEC were treated with concen- trations of glucose at 5.5, 33 mmol/L and glucose at 33 mmol/L with aspirin at 0. 01,0. 1, 1 and 3 mmol/L for 48 h. SA-β-gat staining was used to evaluate senescence. Telomerase activity was detected by PCR-ELISA. Cell cycle and the level of reactive oxygen species （ROS） were detected by flow cytometry. Results Endothelial ceils exhibited the characteristic of senescence, increased the number of β-gal positive cells, decreased telomerase activity significantly, en- hanced the proportion of HUVEC in the G0/G1 phase and reduced that in the S phase and increased the level of ROS after exposure to high glucose （33 mmol/L, P 〈 0.05）. All these phenomena were reversed by 0. 01,0. 1 and 1 mmol/L as pirin remarkably （P 〈 0. 05 ）. However, there was no significant difference between 3 mmol/L aspirin group and high glucose group. Conclusions 0.01, 0. 1 and 1 mmol/L aspirin delay endothelial cell senescence exposed to high glu- cose. This positive effect may be associated with decreasing oxidative stress.

关 键 词：阿司匹林 内皮细胞衰老 端粒酶 细胞周期 活性氧 

分 类 号：R363[医药卫生—病理学]