高分子液态氟碳微球增效高强度聚焦超声治疗兔乳腺VX_2移植瘤实验研究  

Increased Efficacy of Perfluorohexane Encapsulated PLGA Microcapsules For High Intensity Focused Ultrasound for Breast Cancer Ablation

作　　者：王敏[1] 冉海涛[1] 何敏瑜[1] 肖洋[1] 王琦[2] 王志刚[1] 

机构地区：[1]重庆医科大学超声影像学研究所,重庆医科大学附属第二医院超声科,重庆市400010 [2]重庆医科大学生物医学工程学院

出　　处：《中国超声医学杂志》2014年第2期167-170,共4页Chinese Journal of Ultrasound in Medicine

基　　金：国家重点基础研究发展计划(973计划)(No.2011CB707900);国家自然基金重点项目(No.81130025);重庆市高校创新团队计划(No.KJTD201303)

摘　　要：目的观察高分子液态氟碳微球对高强度聚焦超声(HIFU)治疗兔乳腺VX_2移植瘤的增效作用,初步探讨其增效机制。方法制备高分子液态氟碳微球,选取成功建模3周后的荷瘤兔VX_2乳腺移植瘤的新西兰大白兔24只,随机等分为3组,局部注射微球后用HIFU对兔乳腺肿瘤进行辐照。A组为HIFU+生理盐水组,B组为HIFU+高分子微球组,C组为HIFU+高分子液态氟碳微球组,辐照后观察各组辐照区灰度值变化、肿瘤凝固性坏死体积及肿瘤增殖细胞核抗原(PCNA)表达情况,计算增殖指数(PI)。结果 B、C组见到辐照区域灰度值明显变化,A组部分见到灰度变化,C组辐照前后灰度变化面积和变化值与A、B组比较有显著差异(P<0.05),C组肿瘤内的凝固性坏死体积明显大于A、B组(P<0.05),PCNA表达情况少于A、B组,增殖指数与A、B组比较有显著差异(P<0.05)。结论自制的高分子液态氟碳微球能够增效HIFU对肿瘤的治疗作用,增效机制可能与其改变组织声环境、增加超声的空化作用有关,具体作用机制有待深入研究探讨。Objective To observe the increased efficacy of HIFU ablation tumors using Perfluorohexane Encapsulated PLGA microcapsules and explore the synergistic mechanism.Methods Firstly,the Perfluorohexane Encapsulated PLGA microcapsules were prepared.24 New Zealand white rabbits borne VX2 tumors in breast for 3 weeks were randomly divided into three groups and underwent focused ultrasound irradiation.A group（the control group） was irradiated after the injection of saline locality,B group was irradiated after the injection of PLGA microcapsules,C group was irradiated after the Perfluorohexane Encapsulated PLGA microcapsules injection.The gray changes of irradiation areas and coagulation necrosis in tumors were observed,and the Proliferation Index（PI） was calculated.Results Obvious gray change can be seen in all of group A and B,but partly in group A.There were significant differences in gray change values and squares among group C and A,B （P＜0.05）.Coagulative necrosis with round shape and clear boundary was observed in every group immediately after irradiation,and the range of necrosis and PI in group C were much larger than those in group A and B （P＜0.05）.Conclusions The Perfluorohexane Encapsulated PLGA microcapsules can increase the therapeutic efficacy of HIFU.The effect may be due to the microcapsules changed the sound environment of tissue,and increased the cavitation of focused ultrasound.The exact mechanism needs further investigation.

关 键 词：高强度聚焦超声 液态氟碳 肿瘤 

分 类 号：R737.9[医药卫生—肿瘤] R445.1[医药卫生—临床医学]