南林895杨树GmNHX1基因的转入及其耐盐性分析  

Expression of GmNHX1 gene in transgenic Populus deltoides × P. euramericana ‘Nanlin895'and its salt tolerance analysis

作　　者：孙伟博[1] 邓大霞 杨立恒[1] 诸葛强[1] 

机构地区：[1]南京林业大学林木遗传与生物技术省部共建教育部重点实验室,江苏南京210037

出　　处：《福建农林大学学报（自然科学版）》2014年第1期34-38,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：林业公益性行业科研重大专项资助项目(201304102);国家863计划资助项目(2013AA102703);江苏高校优势学科建设工程资助项目

摘　　要：将大豆中编码Na+/H+离子逆向转运蛋白的GmNHX1基因构建到植物表达载体pGWB402Ω,通过农杆菌侵染叶盘法转化南林895杨树(Populus deltoides×P.euramericana cv.‘Nanlin895’),PCR检测和Southern杂交结果表明,GmNHX1基因已经整合入南林895杨树基因组.对转GmNHX1基因的南林895杨树耐盐性的生理生化指标进行检测,结果表明GmNHX1基因的表达能够提高转基因杨树的耐盐性.C, ene GmNHX1was constructed, which cedes Na ＋/H ＋ exchange protein to the expression vector pGWB402Ω of plant. The gene was introduced into NanLin895 Populus by Agrobacterium tumefacien infection, and transgenic plants were detected by PCR and Southern hybridization. The restdt showed that specific hybridization bands were obtained in positive transgenic plants, which suggested that Gm NHX1 gene has been transfenredinto NanLin895 Populus genome. The results of physiological determination showed that the salt tolerance of GnNHX1 transgenic NL895 plants were significantly better than that of the control, which suggested that the expression of C, mNHX1 could enhance the salt tolerance of NanLin895 Populus.

关 键 词：GM NHX1 南林895杨树 转基因 盐胁迫 

分 类 号：S722.5[农业科学—林木遗传育种]