经皮三叉神经电刺激减轻戊四氮诱导的大鼠癫痫发作并抑制海马内IL-1β和TNF-α的表达  

Pretreatment with external trigeminal nerve electrostimulation inhibits seizures and decreases expression of IL-1β and TNF-α in hippocampus with status epilepticus induced by pentylenetetrazol in rats

作　　者：刘益民[1] 王倩倩[2] 贺慧艳[2] 王玉[2] 

机构地区：[1]绍兴第二医院神经内科,浙江绍兴312000 [2]安徽医科大学第一附属医院神经内科,安徽合肥230022

出　　处：《中国病理生理杂志》2014年第2期323-327,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81271444);安徽省卫生厅医学科学研究基金资助项目(No.09B140)

摘　　要：目的:探讨经皮三叉神经电刺激预处理对戊四氮(PTZ)诱发的急性癫痫大鼠的行为学及海马致痫细胞因子白细胞介素1β(IL-1β)及肿瘤坏死因子α(TNF-α)的影响。方法:动物随机分为对照组、致痫组(PTZ组)和经皮三叉神经电刺激组,分别给予7 d、14 d和28 d的假刺激和三叉神经电刺激预处理后,腹腔注射PTZ建立急性癫痫动物模型,观察给药后大鼠癫痫行为学表现,并分别用免疫组织化学方法及ELISA方法对海马IL-1β、TNF-α进行检测。结果:经皮三叉神经电刺激可以明显减轻大鼠的痫性发作级别,减少癫痫发作持续的时间(P<0.05),且海马细胞因子IL-1β及TNF-α的表达明显少于PTZ组(P<0.05或P<0.01)。结论:经皮三叉神经电刺激预处理在PTZ急性点燃癫痫大鼠模型中不仅具有抗惊厥作用,还可以减少海马致痫细胞因子IL-1β及TNF-α的表达,可能为癫痫的防治带来新的策略。AIM： To observe the effect of pretreatment with external trigeminal nerve electrostimulation （eT- NS） on behavioral changes and the expression of interleukin-1β （IL-1β） and tumor necrosis factor α （TNF-α） in hippo- campus of pentylenetetrazol （ PTZ）-treated rats. METHODS ： The rats were randomly divided into control group, PTZ group and eTNS group, and kindled by PTZ after administered 7 d, 14 d and 28 d of consecutive fake electrostimulation or eTNS. Subsequently, the severity and duration of seizure were quantitatively evaluated. The concentrations of IL-1β and TNF-α in hippocampus were detected by the methods of ELISA and immunohistochemisty. RESULTS ： Compared with PTZ group, treatment with eTNS significantly inhibited the severity and duration of seizure （ P 〈 0.05 ）, and significantly re- duced the content of IL-1β and TNF-α in the hippocampus after status epilepticus （ P 〈 0.05 or P 〈 0.01 ）. CONCLU- SION： Pretreatment with eTNS may provide a new approach for prevention and treatment of epileptogenesis.

关 键 词：经皮三叉神经电刺激 白细胞介素1Β 肿瘤坏死因子α 

分 类 号：R742.1[医药卫生—神经病学与精神病学]