FAP和Rho家族蛋白在卵巢癌侵袭迁移中的作用  

FAP and Rho-GTPases on the Invasion and Metastasis of Ovarian Cancer

作　　者：张健[1] 刘洪羽[2] 杨薇薇[1] 李春红[1] 郑芳[1] 蒋淑婉[1] 李云[1] 刘端阳[1] 曲雪梅[1] 陈鹤[1] 

机构地区：[1]哈尔滨医科大学病理教研室,黑龙江哈尔滨150081 [2]齐齐哈尔市第一医院病理科,黑龙江齐齐哈尔161006

出　　处：《现代生物医学进展》2014年第4期624-627,共4页Progress in Modern Biomedicine

基　　金：黑龙江省自然科学基金项目(D201049)

摘　　要：目的:明确FAP是否通过RhoA/ROCK、Rac1-GTP通路发挥促增殖、侵袭和迁移作用。方法:用MTT实验,Transwell实验和迁移实验检测FAP、RhoA/ROCK、Rac1-GTP对卵巢癌细胞系HO-8910PM的增殖,侵袭和迁移的影响。结果:1、MTT法,迁移和侵袭实验证实用Y-27632抑制RhoA/ROCK途径能够促进卵巢癌细胞的增殖、迁移和侵袭,与FAP联合作用时促进作用增强。2、MTT法,迁移和侵袭实验证实NSC23766抑制Rac1途径能够抑制卵巢癌细胞的增殖、迁移和侵袭,与FAP联合作用使FAP的促进作用减弱。结论:1、RhoA/ROCK通路抑制HO-8910PM细胞增殖、迁移和侵袭;Rac1-GTP促进HO-8910PM细胞增殖、迁移和侵袭。2、FAP不是通过RhoA/ROCK而是通过Rac1-GTP信号通路在HO-8910PM细胞发挥促增殖、迁移和侵袭作用的。Objective： To determine whether the FAP promotes the ability of proliferation, invasion and migration of HO-8901PM cells by the RhoA/ROCK, Racl-GTP enzymes. Methods： by detection of FAP using the transwell assay, MTT assay and migration assay, RhoA / ROCK, Racl-GTP on ovarian cancer cell line HO-8910PM proliferation, invasion and migration. Results： 1, MTT, migration and invasion experiments confirmed with Y-27632 inhibited RhoA / ROCK pathway can promote ovarian cancer cell proliferation, migration and invasion, FAP facilitating role was enhanced when combined with Y-27632. 2, MTT method, migration and invasion experiments confirmed NSC23766 inhibited Racl pathway to inhibit ovarian cancer cell proliferation, migration and invasion, the promoting role of FAP was weakened when combined with NSC23766. Conclusions： 1. RhoA / ROCK pathway inhibition of HO-8910PM cell proliferation, migration and invasion; Rac 1-GTP promote HO-8910PM cell proliferation, migration and invasion.2, not by RhoA/ROCK but Racl-GTP signaling pathway, FAP plays a role in HO-8910PM cells proliferation, migration and invasion.

关 键 词：成纤维细胞激活蛋白 肿瘤转移 卵巢癌细胞系HO一8910PM RHOA ROCK Rac1GTP 

分 类 号：R737.31[医药卫生—肿瘤]