扇贝糖胺聚糖对感染HSV-Ⅰ小鼠免疫功能的影响研究  

The Effect of Scallop Skirt Glycosaminoglycan on Immune Function in Mice Infected with HSV-Ⅰ

作　　者：于囡[1] 王海桃 杜鹃[1] 李荣[1] 刘赛[3] 

机构地区：[1]青岛市第八人民医院,山东青岛266100 [2]青岛市中心医院,山东青岛266042 [3]青岛大学,山东青岛266021

出　　处：《现代生物医学进展》2014年第4期668-670,667,共4页Progress in Modern Biomedicine

基　　金：山东省中医药发展科技项目(2005-16)

摘　　要：目的:研究扇贝裙边糖胺聚糖(glycosaminoglycan from Scallop Skirt,SS-GAG)对感染单纯疱疹病毒Ⅰ型(herpes simplex virus type,HSV-Ⅰ)小鼠免疫功能的影响。方法:通过在无菌条件下给予小鼠注射扇贝糖胺聚糖SS-GAG,连续11天,并在给药第三天给小鼠腹腔注射HSV-Ⅰ病毒悬液建立小鼠感染模型,用MTT等方法观察SS-GAG对HSV-Ⅰ感染小鼠腹腔巨噬细胞吞噬活性的影响、对脾脏指数、胸腺指数的影响以及对脾淋巴细胞转化能力等免疫指标的影响。结果:与病毒对照组相比,扇贝糖胺聚糖SS-GAG低剂量组、中剂量组、高剂量组均能显著增强HSV-Ⅰ感染小鼠的腹腔巨噬细胞的吞噬活性和HSV-Ⅰ感染小鼠的脾脏指数和胸腺指数(P<0.01),并且能促进其脾淋巴细胞转化增殖能力(P<0.01)。结论:扇贝糖胺聚糖在体内有一定的抗Ⅰ型单纯疱疹病毒作用。其抗病毒作用可能与增强机体免疫功能有关。Objective： To investigate the effect of scallop skirt glycosaminoglycan on immune fimction in mice infected by HSV-I. Methods： Every mouse was treated by SS-GAG with different doses for 11 days. After 72 hours, the mice were injected by HSV-I. The effects of SS-GAG on the phagocytic activity of peritoneal macrophage and the proliferation of splenic lymphocyte were studied by MTT colorimetry. The index of spleen and thymus were used to study the influences of SS-GAG on fimction of the immune organs. Results： Compared with virus control, SS-GAG （10 mg/kg, 20 mg/kg and 40 mg/kg） could significantly enhance phagocytic activity of peritoneal macrophage in mice infected by HSV-I, and promote proliferation of splenic lymphocyte induced by concanavalina and lipopolysacchari- de （P〈0.01）. Meanwhile, the index of thymus of mice treated by SS-GAG was improved significantly （P〈0.01）. Conclusion： The glycosaminoglycan from scallop skirt （SS-GAG） has the depressant effect on HSV-I in vivo, and the mechanism of anti-virus may attribute to improving the immune function of organs.

关 键 词：扇贝裙边 糖胺聚糖 单纯疱疹病毒I型 免疫调节 

分 类 号：Q95-3[生物学—动物学] R285.5[医药卫生—中药学]