PinX1和hTERT在基底细胞癌、皮肤鳞状细胞癌、日光性角化病皮损中的检测  

Detection of PinX1 and hTERT in the Lesions of Basal Cell Carcinomas,Cutaneous Squamous Cell Carcinoma and Actinic Keratosis

作　　者：方杰[1] 袁伟[1] 陈前明[2] 瓦庆彪[2] 吴波[2] 蔡琦[2] 路永红[2] 周培媚[2] 蒋存火[2] 

机构地区：[1]遵义医学院附属医院皮肤科,贵州遵义563000 [2]成都市第二人民医院皮肤科,四川成都610017

出　　处：《中国皮肤性病学杂志》2014年第3期226-229,233,共5页The Chinese Journal of Dermatovenereology

基　　金：四川省科技支撑计划项目(2010SZ0015)

摘　　要：目的检测基底细胞癌、皮肤鳞状细胞癌及日光性角化病组织中内源性端粒酶抑制基因PinX1和人端粒酶逆转录酶hTERT的水平。方法用免疫组化SP法分别检测PinX1和hTERT蛋白在30例基底细胞癌、25例皮肤鳞状细胞癌、21例日光性角化病及17例正常皮肤组织中的表达。结果 PinX1蛋白在基底细胞癌、皮肤鳞状细胞癌及日光性角化病组织中的阳性表达率明显低于正常组,差异有统计学意义(P均<0.05);基底细胞癌与日光性角化病、鳞状细胞癌组织PinX1蛋白阳性表达率差异无统计学意义(P均>0.05);鳞状细胞癌组织PinX1蛋白阳性表达率低于日光性角化病,差异有统计学意义(P<0.05)。hTERT蛋白在基底细胞癌、皮肤鳞状细胞癌及日光性角化病组织中的阳性表达率明显高于正常组,差异有统计学意义(P均<0.05);基底细胞癌、皮肤鳞癌及日光性角化病组织之间hTERT蛋白阳性表达率差异均无统计学意义(P均>0.05)。基底细胞癌、皮肤鳞状细胞癌及日光性角化病组织中PinX1和hTERT蛋白的阳性表达呈负相关(r=-0.451,P<0.05)。结论 PinX1和hTERT蛋白参与了基底细胞癌、皮肤鳞状细胞癌及日光性角化病的发生、发展。二者在抑制细胞凋亡方面可能存在拮抗作用。Objective To detect the expression of PinXl and hTERT in the basal cell carcinomas （BCC） , cutaneous squamous cell carcinoma （CSCC） and actinic keratosis （AKs）. Methods The expression levels of PinX1 and hTERT protein in 30 cases of BCC ,25 cases of CSCC,21 cases of AKs and 17 normal controls were de- tected with immunohistochemical staining. Results The positive rates of PinX1 in BCC, CSCC and AKs groups were significantly lower than that in normal group（ P 〈 0.05 ） ;The positive rates of PinX1 were no sig- nificant differences between BCC and AKs groups （P 〉 0.05 ） ; There were no significant differences in the positive rates of PinX1 between BCC and CSCC groups （ P 〉 0.05 ） ; the positive rates of PinX1 in CSCC group were lower than that in AKs group（P 〈0.05）. The positive rates of hTERT in BCC,CSCC and AKs group were significantly higher than that in normal group（ P 〈 0.05 ） ;. There were no significant differences among BCC, CSCC and AKs groups in the positive rates of hTERT（ P 〉 0.05 ）. There was a significantly neg- ative correlation between PinX1 and hTERT in BCC, CSCC and AKs （ r = - 0.451, P 〈 0.05 ）. Conclusion PinXl and hTERT could play an important role in the development of BCC, CSCC and AKs. PinX1 and hTERT may have antagonistic effects in the process of apoptosis.

关 键 词：PinX1 HTERT 基底细胞癌 皮肤鳞状细胞癌 日光性角化病 

分 类 号：R739.5[医药卫生—肿瘤]