胃癌中葡萄糖神经酰胺合成酶和P-gp的表达及意义  

Significance and expression of glycosylceramide synthase and P-glycoprotein in gastric cancer

作　　者：胡萍萍[1] 房栋[1] 陈淼[2] 陈谦[3] 范钦和[4] 

机构地区：[1]镇江市第二人民医院病理科,镇江212002 [2]镇江市第一人民医院病理科,镇江212002 [3]江苏大学基础医学与医学技术学院组织胚胎学教研室,镇江212002 [4]江苏省人民医院病理科,南京210029

出　　处：《临床与实验病理学杂志》2014年第2期145-149,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：镇江市社会发展课题(sh2011038)

摘　　要：目的分析葡萄糖神经酰胺合成酶(glucosylceramide synthase,GCS)和P-糖蛋白(P glycoprotein,P-gp)在胃癌组织中的表达及相关性,及二者与临床病理特征的关系。方法采用免疫组化SP法检测132例胃癌组织及癌旁组织中GCS和P-gp蛋白的表达;应用实时荧光定量PCR技术检测胃癌组织中GCS和P-gp mRNA的表达。结果 (1)胃癌组织中GCS、P-gp阳性率均高于癌旁组织(P<0.01)。(2)胃癌组织中GCS与P-gp表达呈正相关(P<0.001)。(3)胃癌组织中GCS和P-gp mRNA表达呈正相关(P<0.01)。(4)胃癌中GCS阳性率与肿瘤浸润深度、淋巴结转移以及TNM分期有关(P<0.05);P-gp阳性率与肿瘤大小、分化程度、浸润深度以及TNM分期有关(P<0.05)。结论胃癌组织中GCS不仅和P-gp与多药耐药有关,且可提示肿瘤的浸润、转移。Purpose To explore the role of expression of glycosylceramide synthase （GCS） and P glycoprotein （P-gp） in multidrug re- sisitance of gastric carcinoma, and to analyze their clinicopathologic significance. Methods Immunohistochemical SP method was per- formed to investigate the GCS and P-gp expression in 132 cases of gastric carcinoma and their surrounding normal tissues. Real-time PCR was adopted to analyze the correlation of GCS and P-gp mRNA in gastric carcinoma. Results （ 1 ） The expression of GCS and P- gp was higher in the carcinoma than their surrounding normal tissues （P 〈 0. 01 ）. （2） The positive correlation of GCS and P-gp ex- pression existed in the gastric carcinoma （ P 〈 0. 001 ）. （3） The positive correlation of their mRNA level also existed in the carcinoma （P 〈 0.01 ）. （4） The expression of GCS was positively correlated to infiltration, lymph node metastasis and TNM stages of gastric car- cinoma （ P 〈 0. 05）, while that of P-gp was positively correlated to size, differentiation, infiltration and TNM stages of gastric carcino- ma （P 〈 0. 05 ）. Conclusions The expression of GCS in gastric carcinoma is not only closely related to P-gp and muhidrug resistance but also related to infiltration and metastasis.

关 键 词：胃肿瘤 葡萄糖神经酰胺合成酶 P-糖蛋白 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]