牙釉质结合肽的体外矿化研究  

Study on In Vitro Biomineralization of Enamel-binding Peptide

作　　者：魏蔚[1] 彭周[2] 邓捷[1] 张威[1] 毛靖[1] 

机构地区：[1]华中科技大学同济医院口腔正畸科,武汉430030 [2]华中科技大学材料科学与工程学院,武汉430074

出　　处：《生物医学工程学杂志》2014年第1期132-135,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30970751)

摘　　要：本研究首次报道具有牙釉质结合活性的环肽序列(-CMPQVMPMC-)。在反筛试验中,投入等量牙釉质粉末和相同滴度噬菌体,外壳蛋白pⅢ上表达该展示肽的噬菌体回收量明显高于不含展示肽的野生型噬菌体M13。固相法体外合成该环状七肽以模拟展示肽在噬菌体表面的构象,并利用96孔板体外快速凝胶单扩散系统验证其对钙/磷沉积的影响。矿化开始后的24h内,每隔6h记录各样本在405nm的OD值。各样本的相对浊度表示为所占24h末空白组OD值增量(100%)的百分比。结果显示,牙釉质结合肽(EBP)能明显延迟凝胶中的钙磷成核,且延迟效果与添加的浓度相关。在矿化24h末,含结合肽的各组凝胶内浊度明显低于空白组,但高于BSA组。We present the binding ability of a new peptide （-CMPQVMPMC-） with dental enamel after being evaluated in the present study. Under a standard procedure, the recovery of M13 filamentous phage was greatly enhanced by displaying the peptide in phage coat protein p Ⅲ, Then the cyclic peptide was synthesized using a solid method. The effect of the cyclic peptide in vitro hiomineralization was tested in a single-diffusion microtiter plate gel system. Absorbance at 405 nm of each sample was recorded for 24 h at every 6 h intervals. The relatively increased values of each sample were expressed as percentages relative to the blank group （100%）. The cyclic peptide resulted in a concentration-dependent delayed nucleation. In addition, the overall values of peptide groups at the end of 24 h were lower than those in the control group but much higher than those in the BSA control group.

关 键 词：牙釉质结合肽 生物矿化 单扩散凝胶系统 

分 类 号：R783.1[医药卫生—口腔医学]