根瘤菌BK-20固定化细胞生产L(+)-酒石酸  

Production of L(+)-tartaric acid by immobilized Rhizobium strain BK-20

作　　者：兰翔[1] 鲍文娜[1] 潘海峰[1] 谢志鹏[1] 张建国[1] 

机构地区：[1]浙江大学生命科学学院生物化学研究所,浙江杭州310058

出　　处：《生物工程学报》2014年第2期315-319,共5页Chinese Journal of Biotechnology

摘　　要：顺式环氧琥珀酸水解酶(CESH)是根瘤菌BK-20生产L(+)-酒石酸的关键酶。为提高其生产效率和生产稳定性,首先优化根瘤菌BK-20的产酶条件,然后利用固定化细胞连续生产L(+)-酒石酸。结果显示,优化后游离细胞酶活达(3 498.0±142.6)U/g,较优化前提高643%。固定化细胞酶活达(2 817.2±226.7)U/g,其最适包埋剂、菌体浓度和凝胶浓度分别为海藻酸钠,10%(W/V)和1.5%(W/V)。固定化细胞连续反应10批后,其形状和酶活均无明显改变,单批次转化率达98%以上,具有良好的生产稳定性。The cis-epoxysuccinate hydrolase （CESH） from Rhizobium strain BK-20 is the key enzyme for L（＋）-tartaric acid production. To establish a highly efficient and stable production process, we first optimized the enzyme production from Rhizobium strain BK-20, and then developed an immobilized cell-culture process for sustained production of L（＋）-tartaric acid. The enzyme activity of free cells reached （3 498.0±142.6） U/g, and increased by 643% after optimization. The enzyme activity of immobilized cells reached （2 817.2±226.7） U/g, under the optimal condition with sodium alginate as carrier, cell concentration at 10% （W/V） and gel concentration at 1.5% （W/V）. The immobilized cells preserved high enzyme activity and normal structure after 10 repeated batches. The conversion rate of the substrate was more than 98%, indicating its excellent production stability.

关 键 词：顺式环氧琥珀酸水解酶 L(+)-酒石酸 根瘤菌 优化 细胞固定化 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]