脂联素基因多态性(-11377C/G,-3964A/G,-45T/G)与脑梗死相关性研究  

The association of Adiponectin gene polymorphism (-11377C/G,-3964A/G,-45T/G) and cerebral infarction

作　　者：相波[1] 丁晓洁[2] 张磊[3] 王超[1] 王晓青[1] 潘旭东[4] 

机构地区：[1]青岛大学医学院第二附属医院神经内科,山东青岛266042 [2]青岛大学医学院第二附属医院高压氧科,山东青岛266042 [3]青岛大学医学院第二附属医院检验科,山东青岛266042 [4]青岛大学医学院第一附属医院东院神经内科,山东青岛266042

出　　处：《中风与神经疾病杂志》2014年第2期125-129,共5页Journal of Apoplexy and Nervous Diseases

摘　　要：目的探讨青岛地区汉族人群脂联素(adiponectin,ADIPOQ)基因多态性与脑梗死的相关性。方法病例对照研究,选取372例急性脑梗死患者(发病≤3 d)和416例同期查体者。采用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment lengyh polymorphism,PCR-RFLP)或直接测序法检测ADIPOQ基因多态性。应用多因素logistic回归分析研究基因多态性与脑梗死的相关性。结果 rs266729(-11377 C/G)的基因型分布在脑梗死组和对照组中有统计学差异(P<0.05);在显性、隐性和累加模式下,rs266729的基因型分布在两组中也有统计学差异(P<0.05),rs266729的G等位基因变异能增加脑梗死的易感性(3种模式下OR值分别为:1.35、2.15、2.33)。调整脑梗死各危险因素后,多因素logistic回归分析表明显性模式下rs266729仍与脑梗死易感性相关(P=0.049,OR=1.44)。rs822396(-3964A/G)和rs2241766(-45T/G)的基因型分布在两组中无统计学差异(P>0.05)。单倍型分析显示脑梗死组和对照组无统计学差异。结论 rs266729(-11377 C/G)基因多态性与脑梗死的易感性相关;rs266729的G等位基因变异可能是脑梗死易感性的危险因素。Objective To investigate the relationship between adiponectin （ADIPOQ） gene polymorphism and cere- bral infarction （CI） in Han population of Qingdao area. Methods A case-control study,372 cases of acute CI （ onset ≤3 d） and 416 cases of healthy examination were selected. Polymerase chain reaction-restriction fragment lengyh polymorphism （PCR-RFLP） or direct sequencing assay was used to detect ADIPOQ gene polymorphism. Multiple Logistic regression anal- ysis studyed the association of the ADIPOQ gene polymorphism and CI. Results Genotype distribution of rs266729 （- 11377C/G） was significantly different （P 〈 O. 05 ） in the CI group from control group;and the same result was found even in dominant, recessive and additive mode. The allele G mutation of rs266729 could increase the susceptibility to CI （ the OR values in three modes were respectively：l. 35,2.15,2.33 ）. After adjusting for risk factors of CI, Multiple Logistic regres- sion analysis showed the allele G of rs266729 was still the risk factor for the CI in the dominant mode （ P = O. 049, OR = 1.44）. There was no significant difference between the CI group and control group in genotye distribution of rs822396 （ - 3964A/G） and rs2241766 （-45T/G） （P 〉 0.05 ）. Haploid type analysis showed no statistical difference between CI group and control group（ P 〉 0.05 ）. Conclusion rs266729 （-11377C/G） is associated with the susceptibility to CI;the allele G mutation of rs266729 may be a risk factor for CI susceptibility.

关 键 词：脑梗死 脂联素 基因多态性 单倍型 

分 类 号：R743.3[医药卫生—神经病学与精神病学]