依达拉奉对大鼠延髓缺血后神经元神经胶质细胞及微血管的影响  

The effect of edaravone on neurons,glial cells and microvasculature in medulla oblongata ischemia in rats

作　　者：朱江[1] 郭森[2] 赵亮[1] 高燕军[1] 赵淑敏[3] 杨振军[2] 

机构地区：[1]承德医学院附属医院神经内科,河北承德067000 [2]承德医学院人体解剖学教研室,河北承德067000 [3]承德医学院电镜室,河北承德067000

出　　处：《中风与神经疾病杂志》2014年第2期147-149,共3页Journal of Apoplexy and Nervous Diseases

基　　金：河北省卫生厅资助项目(No.20130340)

摘　　要：目的探索依达拉奉对大鼠延髓缺血后神经元、神经胶质细胞及微血管的影响。方法将Wistar大鼠分为假手术组、实验组、缺血对照组。其中实验组及缺血对照组,分别给予依达拉奉和生理盐水腹腔注射。标本采用HE染色、单宁酸氯化铁染色,对延髓内微血管密度、神经元计数、神经胶质细胞计数进行观察。结果实验组中神经元数量、微血管密度(MVD)减少的程度及神经胶质细胞增加的程度低于缺血对照组。结论在大鼠延髓缺血后依达拉奉治疗具有明显保护作用。Objective To explore the effect of edaravone on neurons, glial cells and microvasculature in medulla ob- longata ischemia in rats. Methods Adult Wistar rats were divided into sham operation group, edaravoue treatment group and ischemia control group. The medulla oblongata ischemia model was established by blocking bilateral vertebral arteries and unilateral common carotid artery. The rats in the edaravone treatment group and ischemia control group respectively re- ceived edaravone and normal saline by intraperitoneal injections. H＆E staining was used to show neurons and glial cells. Mi- crovaseulature was stained with tannic acid-ferric chloride method（ TA-FE method）. The neuron morphological changes were investigated with electron microscope. Results Neurons and microvascular density in edaravone treatment group were more than in ischemia control group, but glial cells were less than that in isehemia group. Conclusion Edaravone exert a protec- tive effect against medulla oblongata ischemia in rats.

关 键 词：依达拉奉 延髓缺血 神经元 神经胶质细胞 微血管 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]