银杏叶提取物对无心跳供肝热缺血损伤保护作用的实验研究  

Experimental study on the preventive effects of ginkgo leaf extract agent against warm ischemic liver injury in the hepatic grafts from non heartbeating donors in pigs

作　　者：韩德恩[1] 张新晨[1] 田素礼[1] 马丽英[2] 杨春娥 刘长振 

机构地区：[1]哈尔滨医科大学附属第二医院普外科,150086 [2]哈尔滨医科大学病理生理教研室

出　　处：《中华器官移植杂志》2001年第1期48-51,共4页Chinese Journal of Organ Transplantation

基　　金：哈尔滨医科大学211基金资助项目

摘　　要：目的　探讨传统中药银杏叶提取物对肝脏热缺血损伤的保护作用。方法　建立猪肝脏在体完全缺血 90min的模型。实验分为 3组 ,对照组 11头动物 ,实验组 (银杏组 ) 10头动物 ,前列腺素E1对照组 (PGE1组 ) 9头动物。PGE1组和银杏组按分组要求分别于缺血前 30min给予PGE1和银杏叶提取物 ;观测肝组织中自由基、肝脏能量代谢、肝功能及肝组织病理改变等。结果　PGE1组和银杏组上述指标都优于对照组 ,银杏叶提取物比PGE1具有更强的抗自由基作用 ,而在其它方面与PGE1相类似。结论　银杏叶提取物是比PGE1更好的热缺血保护剂 。Objective To study the preventive effects of ginkgo leaf extract agent (ginaton injection) against warm ischemic liver injury in the hepatic grafts from non heartbeating donors in pigs. Methods The pigs underwent 1.5 h hepatic vascular exclusion with venovenous bypass. The animals were divided into 3?groups: control group ( n =11), group 2 ( n =10) receiving ginkgo leaf extract agent and group 3 ( n =9) given PEG1. Agents were given to the pigs by continuous intravenous infusion for 30 min before ischemia. The therapeutic effects were evaluated by test of free radicals, liver function test, blood and tissue biochemistry, and histological analyses. Results The above mentioned parameters in the group 2 and group 3 were significantly improved as compared with those in the control group. But ginaton had stronger anti free radical effect than PGE1. Conclusion Gingko leaf extract agent, which can attenuate warm ischemic liver injury, is a better warm ischemia preventive agent than PGE1.

关 键 词：银杏叶提取物 肝缺血 热缺血损伤 再灌注损伤 抗氧化药 

分 类 号：R965[医药卫生—药理学]