肿瘤坏死因子-α在中耳胆脂瘤骨质破坏中的作用  

The study of TNF-α destructive action to the bone in human middle cholesteatoma

作　　者：薛秋红[1] 金康业[2] 宋海兰[1] 

机构地区：[1]武汉科技大学医学院耳鼻咽喉科,武汉430062 [2]湖北医科大学附属第二医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉科杂志》2001年第1期13-14,I001,共3页Journal of Clinical Otorhinolaryngology

摘　　要：目的 :研究肿瘤坏死因子 - α(TNF- α)在中耳胆脂瘤组织中的表达 ,探讨其在中耳胆脂瘤骨质破坏中的作用。方法 :采用免疫组织化学 SABC染色法和计算机图像定量分析法 ,对 2 5例中耳胆脂瘤和 10例正常外耳道皮肤中 TNF- α的表达情况进行观察。结果 :TNF- α不仅在胆脂瘤上皮的各层细胞胞浆中 ,而且在皮下组织的炎性细胞中都有高度表达。定量分析显示胆脂瘤上皮中 TNF- α的含量显著高于外耳道皮肤 (P <0 .0 5 )。结论 :TNF-α可能是胆脂瘤引起骨质破坏的一个因素。Objective:To study the expression of TNF α in the cholesteatoma and elucidate the role of TNF α in the destruction of bone.Method:All samples (5 μm paraffin sections) from 25 cholesteatoma cases and 10 normal cases were examined by immunohistochemical SABC method and analysed by computer image.Result:In 25 cholesteatoma cases,TNF α expressed in the cytoplasm of the full epithelial tissue cells,subcutaneous inflammatory cells and fibroblast.In contrast,the normal cases,there were 6 cases week positive,4 cases negative.The results of the computer image quantitative analysis system showed that the mean optical density of TNF α was 0.1326 ± 0.0022 in the cholesteatoma epithelial tissue and 0.0868 ± 0.0014 in normal skin epithelial tissue respectively,both of which had significantly differentiation (P< 0.05 ).Conclusion:TNF α may be a factor of the destruction of bone of cholesteatoma.

关 键 词：中耳胆脂瘤 肿瘤坏死因子-A 骨质破坏 

分 类 号：R739.61[医药卫生—肿瘤] R764.23[医药卫生—临床医学]