红毛五加多糖诱导体外人胃癌细胞凋亡的研究  

Gastric Cancer Cell Apoptosis Induced by Acanthopanax Giraldii Harms Var Hispidus Hoo Polysaccharides in Vitro

作　　者：吕晓英[1] 李由[1] 孙菊华 王双印[1] 王维祥[1] 曾令福[3] 

机构地区：[1]成都军区联勤部军事医学研究所,650032 [2]新疆阿图什市卫生防疫站,845350 [3]华西医科大学,610041

出　　处：《实用癌症杂志》2001年第1期6-8,共3页The Practical Journal of Cancer

基　　金：国家自然科学基金资助(No.39070111)

摘　　要：目的　探索红毛五加多糖的体外抑癌机制。方法　采用体外细胞培养方法观察细胞形态学改变 ,采用流式细胞术检测细胞凋亡百分比及细胞周期改变。结果　红毛五加多糖使胃癌细胞出现凋亡的形态学改变。该多糖作用后在细胞周期直方图上出现凋亡峰 ,0 .1mmol/L红毛五加多糖样品Ⅱ (AGPⅡ )作用 36h、红毛五加多糖样品Ⅲ (AGPⅢ )作用 48h后 ,凋亡细胞分别占11.2 %和 15 .5 % ,并使细胞周期明显阻滞于G0 /G1期 ,具有时间—效应关系 ,且AGPⅡ的作用效果较AGPⅢ更为明显。结论　该多糖的抑癌机制与诱导细胞凋亡作用有关。Objective To investigate the possible mechanisms of Acanthopanax giraldii Harms Var Hispidus Hoo polysaccharides(AGP) on the human gastric cancer cells in vitro.Methods In vitro culture was used to observe the morphological changes of the cancer cells and flow cytometry was adopted to explore the percentage of apoptotic cells and cell cycle.Results Apoptotic morphological changes were observed after SGC 7901 cultured with AGP.After cultured with 0.1 mmol/L AGPⅡ for 36 h,AGPⅢ for 48 h,apoptotic cell percentages were 11.2% and 15.5%,respectively.There were apoptotic peaks in cell cycle analysis,and AGP made the tumor cell stopping at G 0/G 1 phase,with a time effects relationship.Also,the effects of AGPⅡ were supurior to those of AGP Ⅲ.Conclusion The mechanisms of AGP antitumor effects may be associated with apoptosis induction.

关 键 词：红毛五加多糖 胃癌 凋亡 

分 类 号：R735.2[医药卫生—肿瘤] R73－361[医药卫生—临床医学]