肝前型门静脉高压症动物(犬)模型制作体会  

Experiences in making canine models of pre-hepatic portal hypertension

作　　者：赵莉[1] 李振东[1] 于增文[1] 孙静生[1] 仲智勇[1] 

机构地区：[1]石家庄河北医科大学第二医院,050000

出　　处：《中华小儿外科杂志》2001年第1期42-44,共3页Chinese Journal of Pediatric Surgery

基　　金：河北省自然科学基金资助项目!(编号 395 42 3)

摘　　要：目的　为使肝前型门脉高压症动物实验更可靠 ,建立符合或接近其临床表现的动物模型。方法　实验犬 2 9只。开腹测脾体积、门静脉直径及压力。部分结扎门脉使其截面积缩小95 .0 % ,门脉压上升到结扎前的 3倍 ,胃肠无明显淤血时关腹。分别在术后 2、4、8、12周及 16周再次开腹观察胃肠淤血及侧支循环情况 ,测门脉压及脾体积。结果　 4只死亡。 2～ 8周的门脉压较高 ,腹膜后水肿、肠壁肥厚。随着侧支循环的建立 ,压力逐渐下降 ,12～ 16周门脉压稳定 ,腹膜后水肿消失 ,交通血管清晰。 12、16周门脉压低于 2、4周 (P <0 .0 5 ) ;12周与 16周的门脉压力比较P >0 .0 5。结论　采用部分结扎门静脉的方法制作肝前型门脉高压症动物模型 ,缩窄门脉后 12～ 16周为成功 ;更接近其临床表现 ,有一定的侧支循环的门脉高压症 。Objective To insure the reliability of animal models of pre hepatic portal hypertension.Methods The size of spleen, diameter and pressure of portal vein of 29 canine models were measured. A partial ligation of portal vein was performed narrowing at 95% of its original cross section, or making the portal vein pressure more than three times to its original pressure and the gastrointestinal venous stasis could not be seen. The features of gastrointestinal venous stasis and collateral circulation were observed during reoperation on the 2nd, 4th, 8th, 12th and 16th weeks postoperatively.Results Four dogs died from abdominal infection, anesthesia and acute enteric necrosis respectively. The higher pressure of portal vein, retroperitoneal edema, hypertrophy of the intestinal wall were noted. Following the formation of collaterals between portal and systemic circulation , the portal vein pressure decreased gradually from 2nd to 8th weeks. From 12th to 16th weeks postoperatively, the portal vein pressure was stabilized but still higher than that of the normal, retropertioneal edema disappeared and collateral vessels could be clearly seen. The pressure of portal vein in the 12th, 16th weeks was lower than that in 2nd, 4th weeks ( P < 0.05 ). Conclusions Making animal models of prehepatic portal hypertension by narrowing the diameter of portal vein is a reliable method. The status of models in 12th～16th weeks after operation are similar to children with portal hypertension.

关 键 词：肝前型门静脉高压症 疾病模型 制作 

分 类 号：R-332[医药卫生]