抗人食管癌单克隆抗体在荷瘤裸鼠体内的定位  

Study of Location in Tumor Bearing Nude Mice of Monoclonal Antibody to Human Esophageal Carcinoma

作　　者：刘长征[1] 何军芳[1] 梁昌盛[1] 林鹏[2] 高锦辉[1] 刘乐和[3] 沈忠英[4] 

机构地区：[1]中山医科大学实验核医学教研室,广东广州510089 [2]中山医科大学肿瘤医院,广东广州510089 [3]中山医科大学微生物教研室,广东广州510089 [4]汕头大学医学院病理教研室,广东汕头515031

出　　处：《中山医科大学学报》2001年第1期19-21,I001,共4页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：国家自然科学基金项目! (3 0 0 70 2 3 1) ;广东省自然科学基金项目! (960 777)

摘　　要：【目的】研究抗人食管癌单克隆抗体G9(McAbG9)在荷瘤裸鼠体内对食管癌组织的定位作用。【方法】采用氯胺 T法制备标记物12 5I G9,纯化后腹腔注射 ,分别于 2 4、48、72h取多种器官 /组织测放射性 ,计算每克组织摄取放射性的百分比(%ID/ g)及肿瘤与非瘤组织的比T/NT ,并取 72h的肿瘤组织进行放射自显影分析。【结果】12 5　I G9肿瘤组织的放射性均较其它器官 /组织高 (血液除外 ) ,48h组最高 ,T/NT值在 2～ 7之间 ;放射自显影显示肿瘤组织有大量放射性浓集 ,肿瘤边缘较中心部位明显。【结论】12 5　I G9能在荷瘤裸鼠体内定位于食管癌部位 ,单抗G9具有很好的导向作用。Objective To study the location effect of the McAb G9 to human esophageal carcinoma in tumor bearing nude mice. Methods 125 I-G9 was prepared by Chloramine-T method. The distribution of 125 I-G9 was detected at different time (24, 48, and 72 h) after peritoneal injection. The percentage of the injected dose per gram of tissue(%ID/g) and the ratio of Tumor/non-Tumor were calculated. Results The distribution of 125 I-G9 in tumor at the third day was showed by autoradiography obviously higher than in other organ/tissue (except blood) and the highest is at the 48 h. The T/NT values are 2-7. The autoradiography indicated that radioactivity concentrated in tumor tissue. The concentration in tumor edge is more obvious than in the center. Conclusion 125 I-G9 has a considerable targeting activity and can well locate in esophageal carcinoma tissue in tumor bearing nude mice.

关 键 词：食管癌 诊断 抗人食管癌单克隆抗体 定位 碘125 

分 类 号：R735.1[医药卫生—肿瘤] R730.44[医药卫生—临床医学]