血浆miR-125a-5p在肝纤维化患者中的表达及意义  被引量:2

miR-125a-5p expression and its clinical significance in liver fibrosis patients

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作  者:林德照 郑建建[2] 林镯[2] 董培红[2] 俞富军[2] 

机构地区:[1]温州市中医院普外科,浙江温州325000 [2]温州医科大学附属第一医院,浙江温州325000

出  处:《中国卫生检验杂志》2014年第3期380-382,共3页Chinese Journal of Health Laboratory Technology

基  金:国家自然科学基金(81000176/H0317;81100292/H0317);浙江省自然科学基金(Y2090326;Y2110634);王宝恩肝纤维化研究基金(20100002;20120127);温州市科技局资助项目(Y20110033;Y20120127)

摘  要:目的探讨血浆miR-125a-5p在肝纤维化患者中的表达及意义。方法收集50例健康志愿者及130例慢性乙型肝炎患者的血浆进行miR-125a-5p实时荧光定量聚合酶链反应(real-time PCR)检测。结果相对于正常组,肝纤维化S1-S4组的miR-125a-5p显著增加(P<0.05),且纤维化越严重,miR-125a-5p的表达量就越高(P<0.05)。患者组中肝硬化患者经扶正化瘀等治疗后,治疗后第7 d及第30 d复查miR-125a-5p表达量较治疗前明显减低(P<0.05),且随着治疗的延长,miR-125a-5p的表达表现为持续下降(P<0.05)。相关性分析显示miR-125a-5p表达与child-pugh分级呈现出高度正相关(相关系数r=0.841,P<0.01)。深入研究显示,患者组中HBeAg(+)患者miR-125a-5p表达量明显高于HBeAg(-)患者(P<0.05)。结论血浆miR-125a-5p的测定有助于肝纤维化的诊断及分期,并对肝硬化患者的病情估计及预后治疗有着一定的帮助。Objective To discuss the clinical value of miR - 125a -5p expression in liver fibrosis patients. Methods The lev- els of miR - 125a -5p in the plasma of 50 healthy controls and 130 chronic hepatitis B patients were measured by real -time fluo- rescent quantitative PCR. Results The levels of miR - 125a - 5p were significantly higher in liver fibrosis S1 - S4 groups com- pared with the healthy controls( P 〈0.05 ). With the development of liver fibrosis, the expression levels of miR - 125a -5p in- creased( P 〈0.05 ). In hepatic cin'hosis patients, the levels of miR - 125a -5p obviously decreased after Fuzheng Huayu therapy in the 7th day and the 30lh clay( P 〈0.05 ). With the further treatment, the curative effect of the 30'h day was better than that of the 7th day (P 〈 0.05 ). miR- 125a- 5p expression showed highly positive correlation with child- pugh classification (r : 0.841, P 〈0.01 ). The further study indicated the expression levels of miR - 125a -5p in HBeAg( + ) patients were higher than those in HBeAg ( - ) patients (P 〈 0.05 ). Conclusion The plasma level detection of miR - 125a -5p is helpful to diagnosis and clinical staging of liver fibrosis, as well as evaluation of the disease progression and prognosis of hepatic cirrhosis patients.

关 键 词:肝纤维化 miR-125a-5p 实时定量PCR 

分 类 号:R575.2[医药卫生—消化系统]

 

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